AbstractIn this work, an innovative and fast analytical method for the quantification of soyasaponins I andβgin lentils has been developed. Samples were extracted using 70 % aqueous ethanol at room temperature and then injected into a high-performance liquid chromatography–tandem mass spectrometry system. The correlation coefficients of calibration curves of the analyzed compounds were ≥0.9997. The recoveries obtained by spiking the lentil samples with a standard mixture of soyasaponins I andβgat50and100mgl −1 were in the range of 96–101 and 98–103 %, respectively. The validated method was applied to the analysis of 30 lentil samples from central Italy. Soyasaponins I andβgwerepresent in these lentils in concentrations that ranged from 54 to 226 mg kg −1 and from 436 to 1,272 mg kg −1 ,respectively. Our data indicated that lentils cultivated in fields at intermediate altitudes (1,142–1,387 m) showed the highest levels of soyasaponins, a finding confirmed by principal component analysis.
Rapid Quantification of Soyasaponins I and βg in Italian Lentils by High-Performance Liquid Chromatography (HPLC)-Tandem Mass Spectrometry (MS/MS)
CAPRIOLI, GIOVANNI;CONTI, Paolo;MAGGI, Filippo;RICCIUTELLI, Massimo;TORREGIANI, Elisabetta;VITTORI, Sauro;SAGRATINI, Gianni
2014-01-01
Abstract
AbstractIn this work, an innovative and fast analytical method for the quantification of soyasaponins I andβgin lentils has been developed. Samples were extracted using 70 % aqueous ethanol at room temperature and then injected into a high-performance liquid chromatography–tandem mass spectrometry system. The correlation coefficients of calibration curves of the analyzed compounds were ≥0.9997. The recoveries obtained by spiking the lentil samples with a standard mixture of soyasaponins I andβgat50and100mgl −1 were in the range of 96–101 and 98–103 %, respectively. The validated method was applied to the analysis of 30 lentil samples from central Italy. Soyasaponins I andβgwerepresent in these lentils in concentrations that ranged from 54 to 226 mg kg −1 and from 436 to 1,272 mg kg −1 ,respectively. Our data indicated that lentils cultivated in fields at intermediate altitudes (1,142–1,387 m) showed the highest levels of soyasaponins, a finding confirmed by principal component analysis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
