An on-field survey was conducted to assess the physiological response related to polyphenol content and antioxidant activity of S. pallida grown wild on Cambisol in an abandoned mining site in Northeast Morocco. Results indicated that S. pallida maintained high symbiotic efficiency even at late growth stages, while accumulating significant levels (mg kg–1 DM) of Fe (4270), Pb (1010), Zn (693), and Cu (129) in its aerial parts. Phenol content varied significantly (p < 0.05) by phenological stage and plant part, with a high concentration of total extractable polyphenols (7.73% DM) and tannin compounds (6.97% DM) recorded during the vegetative stage. Antioxidant activity, as shown by DPPH radical scavenging (IC50 of 0.126 mg mL–1), was comparable to the control (BHT, IC50 of 0.110 mg mL–1). Anti-lipid peroxidation and ferric reduction potential were higher during the flowering stage (IC50 of 0.356 and 0.112, respectively), with values exceeding those of the controls (0.560 and 0.310). Finally, HPLC-ESI-MS/MS analysis identified 38 compounds, with high levels (mg kg–1 dried extract) of 4-hydroxybenzoic acid (401.30), rutin (313.51), vanillic (245.26), gallic (243.44), and p-coumaric acids (209.29). These findings highlighted S. pallida as a promising legume species for restoring heavy metal-contaminated pastures and promoting sustainable land use and fertility.
Quantification of Bioactive Compounds and Evaluation of Antioxidant Activity in Sulla pallida Desf. from Mining Sites of Morocco: Implications for the Restoration of Heavy Metal-Contaminated Soils
G. Caprioli;L. Acquaticci;
2025-01-01
Abstract
An on-field survey was conducted to assess the physiological response related to polyphenol content and antioxidant activity of S. pallida grown wild on Cambisol in an abandoned mining site in Northeast Morocco. Results indicated that S. pallida maintained high symbiotic efficiency even at late growth stages, while accumulating significant levels (mg kg–1 DM) of Fe (4270), Pb (1010), Zn (693), and Cu (129) in its aerial parts. Phenol content varied significantly (p < 0.05) by phenological stage and plant part, with a high concentration of total extractable polyphenols (7.73% DM) and tannin compounds (6.97% DM) recorded during the vegetative stage. Antioxidant activity, as shown by DPPH radical scavenging (IC50 of 0.126 mg mL–1), was comparable to the control (BHT, IC50 of 0.110 mg mL–1). Anti-lipid peroxidation and ferric reduction potential were higher during the flowering stage (IC50 of 0.356 and 0.112, respectively), with values exceeding those of the controls (0.560 and 0.310). Finally, HPLC-ESI-MS/MS analysis identified 38 compounds, with high levels (mg kg–1 dried extract) of 4-hydroxybenzoic acid (401.30), rutin (313.51), vanillic (245.26), gallic (243.44), and p-coumaric acids (209.29). These findings highlighted S. pallida as a promising legume species for restoring heavy metal-contaminated pastures and promoting sustainable land use and fertility.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
