Pulses are an important source of proteins, carbohydrates, and dietary fibre, and also contain polyphenols, which are bioactive compounds with antioxidant activity. A new analytical method that uses high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) was developed for the quantification of sixteen polyphenols in thirty-one pulse varieties. Different extraction procedures were tested (acidic hydrolysis, alkaline hydrolysis and extraction without hydrolysis), and acidic hydrolysis at pH 2, extraction temperature of 20 °C and extraction time of 2 h was proven to be the best in terms of recovery percentages (99.7–107.6%). The highest polyphenol levels were found in beans, particularly black beans (459 mg kg−1) and ruviotto beans (189 mg kg−1); significant levels of polyphenols were also observed in lentils, particularly black lentils (137 mg kg−1) and quality gold lentils (132 mg kg−1). This study provides new information about legume polyphenols, offering reasons to promote legumes as part of a healthy diet.
Optimization of an extraction method for the simultaneous quantification of sixteen polyphenols in thirty-one pulse samples by using HPLC-MS/MS dynamic-MRM triple quadrupole
Giovanni Caprioli;Franks Kamgang Nzekoue;Sauro Vittori;Gianni Sagratini
2018-01-01
Abstract
Pulses are an important source of proteins, carbohydrates, and dietary fibre, and also contain polyphenols, which are bioactive compounds with antioxidant activity. A new analytical method that uses high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) was developed for the quantification of sixteen polyphenols in thirty-one pulse varieties. Different extraction procedures were tested (acidic hydrolysis, alkaline hydrolysis and extraction without hydrolysis), and acidic hydrolysis at pH 2, extraction temperature of 20 °C and extraction time of 2 h was proven to be the best in terms of recovery percentages (99.7–107.6%). The highest polyphenol levels were found in beans, particularly black beans (459 mg kg−1) and ruviotto beans (189 mg kg−1); significant levels of polyphenols were also observed in lentils, particularly black lentils (137 mg kg−1) and quality gold lentils (132 mg kg−1). This study provides new information about legume polyphenols, offering reasons to promote legumes as part of a healthy diet.File | Dimensione | Formato | |
---|---|---|---|
polyphenols legumes triple quadr ms ms.pdf
solo gestori di archivio
Tipologia:
Versione Editoriale
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
256.46 kB
Formato
Adobe PDF
|
256.46 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.