The separation and quantitation of the six isomers of pyridinedicarboxylic acids (PDAs) can be achieved rapidly utilizing High Pressure Liquid Chromatography (HPLC). Optimal separation is accomplished using reverse phase chromatography with an aqueous mobile phase mantained at a pH of 7.3 by 153.2 mM phosphate buffer, containing 15 mM tetrabutylammonium phosphate as ion-pairing agent and 2 mM EDTA as mobile phase additive. The influence of the eluent parameters on retention of PDAs has been investigated in order to elucidate the separation mechanisms involved in the ion pair chromatography of these ionizable substances.
Separation of all isomers of pyridinedicarboxylic acids by ionpairing chromatographhy
PASSAMONTI, Paolo;PUCCIARELLI, Filippo
1997-01-01
Abstract
The separation and quantitation of the six isomers of pyridinedicarboxylic acids (PDAs) can be achieved rapidly utilizing High Pressure Liquid Chromatography (HPLC). Optimal separation is accomplished using reverse phase chromatography with an aqueous mobile phase mantained at a pH of 7.3 by 153.2 mM phosphate buffer, containing 15 mM tetrabutylammonium phosphate as ion-pairing agent and 2 mM EDTA as mobile phase additive. The influence of the eluent parameters on retention of PDAs has been investigated in order to elucidate the separation mechanisms involved in the ion pair chromatography of these ionizable substances.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
