This in vitro study was carried out to evaluate the potential antibacterial properties of canine non transfusional hemo-components. Therapeutic formulation commonly used for regenerative medicine purposes (platelet-rich plasma, platelet gel, platelet lysate, fibrin glue), considering both leukocytes-rich and leukocytes-poor formulations, but also platelet-poor plasma and activating substances (thrombin, calcium gluconate), were tested to detect elements with potential antimicrobial properties. The antibacterial effect was tested on different bacterial strains (Staphylococcus aureus subspecies aureus, Staphylococcus cohnii subspecies cohnii, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae subspecies pneumoniae) isolated from canine wounds and classified as sensitive, multidrug-, extensively-, and pandrug-resistant bacteria towards a known panel of human and veterinary antibiotics. The evaluation was carried out by agar gel diffusion method (Kirby-Bauer) and micro-inhibition in broth using microplates and spectrophotometer reading. The study findings confirmed the hypothesized antibacterial properties of canine non transfusional hemo-components. A more effective bacteriostatic effect was found against Gram negative bacteria, drug-resistant too. The presence of leukocytes or platelets does not appear to be essential for the antibacterial effect. Further studies should be conducted to evaluate the exact mechanism of action of the antimicrobial activity. However, non transfusional hemo-components could be a useful natural aid in controlling bacterial infections in dogs.

Antibacterial properties of canine platelet-rich plasma and other non transfusional hemo-components: an in vitro study

Anna-Rita Attili
Primo
;
Evelina Serri;Vincenzo Cuteri;Martina Linardi;Giacomo Rossi;Livio Galosi;Adolfo Maria Tambella
Ultimo
2021-01-01

Abstract

This in vitro study was carried out to evaluate the potential antibacterial properties of canine non transfusional hemo-components. Therapeutic formulation commonly used for regenerative medicine purposes (platelet-rich plasma, platelet gel, platelet lysate, fibrin glue), considering both leukocytes-rich and leukocytes-poor formulations, but also platelet-poor plasma and activating substances (thrombin, calcium gluconate), were tested to detect elements with potential antimicrobial properties. The antibacterial effect was tested on different bacterial strains (Staphylococcus aureus subspecies aureus, Staphylococcus cohnii subspecies cohnii, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae subspecies pneumoniae) isolated from canine wounds and classified as sensitive, multidrug-, extensively-, and pandrug-resistant bacteria towards a known panel of human and veterinary antibiotics. The evaluation was carried out by agar gel diffusion method (Kirby-Bauer) and micro-inhibition in broth using microplates and spectrophotometer reading. The study findings confirmed the hypothesized antibacterial properties of canine non transfusional hemo-components. A more effective bacteriostatic effect was found against Gram negative bacteria, drug-resistant too. The presence of leukocytes or platelets does not appear to be essential for the antibacterial effect. Further studies should be conducted to evaluate the exact mechanism of action of the antimicrobial activity. However, non transfusional hemo-components could be a useful natural aid in controlling bacterial infections in dogs.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/453806
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