The isolation and characterization from the sand flyPhlebotomus perniciosusof aWickerhamomyces anomalusyeast strain (Wa1F1) displaying the killer phenotype was recently reported. In the present work, the killer toxin (KT) produced byWa1F1 was purified and characterized, and its antimicrobial activity in vitro was investigated against fluconazole- susceptible and -resistant clinical isolates and laboratory strains ofCandida albicansandC. glabratadisplaying known mutations.Wa1F1-KT showed a differential killing ability against different mutant strains of the same species. The results may be useful for the design of therapeutic molecules based onWa1F1-KT and the study of yeast resistance mechanisms.
Candidacidal Activity of a Novel Killer Toxin from Wickerhamomyces anomalus against Fluconazole-Susceptible and -Resistant Strains
Bandi, Claudio;Ricci, Irene;Epis, Sara;
2018-01-01
Abstract
The isolation and characterization from the sand flyPhlebotomus perniciosusof aWickerhamomyces anomalusyeast strain (Wa1F1) displaying the killer phenotype was recently reported. In the present work, the killer toxin (KT) produced byWa1F1 was purified and characterized, and its antimicrobial activity in vitro was investigated against fluconazole- susceptible and -resistant clinical isolates and laboratory strains ofCandida albicansandC. glabratadisplaying known mutations.Wa1F1-KT showed a differential killing ability against different mutant strains of the same species. The results may be useful for the design of therapeutic molecules based onWa1F1-KT and the study of yeast resistance mechanisms.| File | Dimensione | Formato | |
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