In this study, a headspace solid-phase microextraction (HS-SPME) method, in combination with gas chromatography flame ionisation detector and gas chromatography–mass spectrometry, has been developed for use in the analysis of the volatile compounds of Teucrium flavum L. subsp. flavum, a plant whose particular fragrance is used in the preparation of flavoured wines, bitters and liqueurs, or as a substitute for hops in the flavouring of beer. The tested fibres were 100 mm poly(dimethylsiloxane) (PDMS), the 65 mm PDMS/divinylbenzene (DVB) and 50/30 mm DVB–carboxen–PDMS. The best fibre was found to be PDMS when working in the following conditions: 60C temperature, 30 min extraction time, 30 mg sample amount, 1mm sample particle size. The HS-SPME method permitted the identification (95.8–97.8%) of 76 (dry) and 66 (fresh) different volatiles. In addition, we discovered that the presence of water in the sample can enhance the absolute quantity of alcoholic compounds such as 1-octen-3-ol and reduce the presence of esters such as methyl geranate.

Analysis of the volatile compounds of Teucrium flavum L. subsp flavum (Lamiaceae) by headspace solid-phase microextraction coupled to gas chromatography with flame ionization and mass spectrometric detection

SAGRATINI, Gianni;MAGGI, Filippo;PAPA, Fabrizio;VITTORI, Sauro
2012

Abstract

In this study, a headspace solid-phase microextraction (HS-SPME) method, in combination with gas chromatography flame ionisation detector and gas chromatography–mass spectrometry, has been developed for use in the analysis of the volatile compounds of Teucrium flavum L. subsp. flavum, a plant whose particular fragrance is used in the preparation of flavoured wines, bitters and liqueurs, or as a substitute for hops in the flavouring of beer. The tested fibres were 100 mm poly(dimethylsiloxane) (PDMS), the 65 mm PDMS/divinylbenzene (DVB) and 50/30 mm DVB–carboxen–PDMS. The best fibre was found to be PDMS when working in the following conditions: 60C temperature, 30 min extraction time, 30 mg sample amount, 1mm sample particle size. The HS-SPME method permitted the identification (95.8–97.8%) of 76 (dry) and 66 (fresh) different volatiles. In addition, we discovered that the presence of water in the sample can enhance the absolute quantity of alcoholic compounds such as 1-octen-3-ol and reduce the presence of esters such as methyl geranate.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11581/213111
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