Surface adsorption of protein corona controls the cell uptake mechanism in efficient cationic liposome/DNA complexes in serum.

Here we apply a recently proposed programmed packaging strategy to develop a novel multicomponent envelope-type nanoparticle system (MENS). To this end, we took specific advantage of using multicomponent liposomes that have recently exhibited intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes and to enter the nucleus with extremely high efficiency. Transfection efficiency experiments on NIH 3T3 mouse fibroblasts indicate that MENS is a promising transfection candidate.