In this paper, we demonstrate that synthetic epidermal mitosis inhibiting pentapeptide (pyroGlu-Glu-Asp-Ser-Gly) is phosphorylated in vitro at serine level by protein kinase NII isolated from calf thymus chromatin. A serum enzyme, which rapidly cleaves the synthetic epidermal mitosis inhibiting pentapeptide, also hydrolyses the synthetic transcription inhibiting pentapeptide (pyroGlu-Ala-Glu-Ser-Asn). The phosphorylated forms of both pentapeptides are protected from the serum enzyme activity.
Epidermal inhibitory pentapeptide phosphorylated in vitro by calf thymus protein kinase NII is protected from serum enzyme hydrolysis.
BRAMUCCI, Massimo;MIANO, Antonino;
1992-01-01
Abstract
In this paper, we demonstrate that synthetic epidermal mitosis inhibiting pentapeptide (pyroGlu-Glu-Asp-Ser-Gly) is phosphorylated in vitro at serine level by protein kinase NII isolated from calf thymus chromatin. A serum enzyme, which rapidly cleaves the synthetic epidermal mitosis inhibiting pentapeptide, also hydrolyses the synthetic transcription inhibiting pentapeptide (pyroGlu-Ala-Glu-Ser-Asn). The phosphorylated forms of both pentapeptides are protected from the serum enzyme activity.File in questo prodotto:
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