Bovine herpesvirus-1 (BHV-1) is responsible for a variety of clinical signs. It is widespread in cattle and causes severe economic losses (Castrucci et al., 2002a, b). To prevent the infection several live and inactivated vaccines are commonly used. However, due to their short-term immunity and incomplete protection, new vaccine strategies have been proposed such as genetic vaccination (Babiuk et al., 1999). With this aim a DNA vaccine, with a plasmid expressing the tgD glycoprotein, known to be responsible for the virus antigenicity and consequent immunogenicity (Castrucci et al., 2004; Gupta et al., 1998), has been investigated. In the present study, the ELISA reaction was performed in order to detect specific antibodies in calves vaccinated with a DNA vaccine using the pcDNA3.1-tgD plasmid.
An Immunoenzyme Linked Assay (ELISA) for the Detection of Antibodies to Truncated Glycoprotein D (tgD) of Bovine Herpesvirus-1
VINCENZETTI, Silvia;VITA, Alberto;AMICI, Augusto;
2006-01-01
Abstract
Bovine herpesvirus-1 (BHV-1) is responsible for a variety of clinical signs. It is widespread in cattle and causes severe economic losses (Castrucci et al., 2002a, b). To prevent the infection several live and inactivated vaccines are commonly used. However, due to their short-term immunity and incomplete protection, new vaccine strategies have been proposed such as genetic vaccination (Babiuk et al., 1999). With this aim a DNA vaccine, with a plasmid expressing the tgD glycoprotein, known to be responsible for the virus antigenicity and consequent immunogenicity (Castrucci et al., 2004; Gupta et al., 1998), has been investigated. In the present study, the ELISA reaction was performed in order to detect specific antibodies in calves vaccinated with a DNA vaccine using the pcDNA3.1-tgD plasmid.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
