Effect of NADH on the redox state of human hemoglobin

Falcioni, Giancarlo; Caulini, G.; Olek, R.; Antosiewicz, J.

In this work, we report that NADH can increase the autoxidation rate of hemoglobin (HbA) in a pH-dependent fashion. During this process, this cofactor is itself oxidized. The presence of superoxide dismutase (SOD) and/or catalase (CAT) can inhibit this result. At lower pH rates, the effect of NADH on the hemoglobin autoxidation rate is more enhanced; in addition, the rate of NADH oxidation is increased. Our data indicates that the reduced pyridine nucleotide may influence the redox state of human hemoglobin by a mechanism, which probably involves free radical species.

Effect of NADH on the redox state of human hemoglobin / FALCIONI G.; CAULINI G.; OLEK R.; ANTOSIEWICZ J.. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 324:1-2(2002), pp. 129-134.

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Titolo:	Effect of NADH on the redox state of human hemoglobin
Autori:	FALCIONI, Giancarlo CAULINI G. OLEK R. ANTOSIEWICZ J. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2002
Rivista:	CLINICA CHIMICA ACTA
Citazione:	Effect of NADH on the redox state of human hemoglobin / FALCIONI G.; CAULINI G.; OLEK R.; ANTOSIEWICZ J.. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 324:1-2(2002), pp. 129-134.
Abstract:	In this work, we report that NADH can increase the autoxidation rate of hemoglobin (HbA) in a pH-dependent fashion. During this process, this cofactor is itself oxidized. The presence of superoxide dismutase (SOD) and/or catalase (CAT) can inhibit this result. At lower pH rates, the effect of NADH on the hemoglobin autoxidation rate is more enhanced; in addition, the rate of NADH oxidation is increased. Our data indicates that the reduced pyridine nucleotide may influence the redox state of human hemoglobin by a mechanism, which probably involves free radical species.
Handle:	http://hdl.handle.net/11581/6557
Appare nelle tipologie:	Articolo

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