MANIFESTATION OF RADIOPROTECTIVE PROPERTIES IN COPPER COMPLEXES [Cu(LCF3)2] AND [Cu(ADM)(PPH3)2]PF6

One of the first and direct signs of ionizing radiation’s effect on a cell is chromosomes destabilization. By studying cytogenetic indices – including mitotic index, chromosomal aberrations, and the number of polyploid cells in the bone marrow cells of the femur, a significant difference was observed between intact and irradiated animals. This suggests that these indices can be considered as markers of irradiation by the technetium isotope. When assessing the survival rate, the highest survival value was recorded in the Group injected with [Сu(Adm)(PPh3)2]PF6. In terms of chromosomal aberrations and number of polyploid cells, irradiated animals showed reliable difference compared to the “irradiation + [Cu(LCF3)2]” group at both 15 and 30 days post-exposure, demonstrating the radioprotective property of the compound. Regarding the mitotic index, treatment with [Cu(LCF3)2] showed a trend toward normalization after 15 days, with a reliable difference between Groups 2 and 3 by the end of the study (after 30 days), further supporting the compound’s beneficial effect. Analysis of survival, changes in cytogenetic parameters, and multivariate regression analysis when using both complexes confirms the highest efficiency of [Сu(Adm) (PPh3)2]PF6 compared to [Cu(LCF3)2]. Specifically, treatment with [Сu(Adm)(PPh3)2]PF6, in contrast to “pure irradiation”, resulted in a significant difference across all parameters and led to the normalization of cytogenetic parameters from the early stages of observation. This suggests a pronounced radioprotective effect of the compound and an accelerated normalization of cytogenetic parameters.