This study aimed to investigate the susceptibility of Trypanosoma brucei to the Anthriscus nemorosa essential oils (EOs), isolated compounds from these oils, and artificial mixtures of the isolated compounds in their conventional and nanoencapsulated forms. The chemical composition of the essential oils from the aerial parts and roots of Anthriscus nemorosa, obtained from a wild population growing in central Italy, were analyzed by gas chromatography/mass spectrometry(GC/MS). In both cases, the predominant class of compounds was monoterpene hydrocarbons, which were more abundant in the EOs from the roots (81.5%) than the aerial parts (74.0%). The overall results of this work have shed light on the biological properties of A. nemorosa EO from aerial parts (EC50 =1.17 mg/mL), farnesene (EC50 = 0.84 mg/mL) and artificial mixtures (Mix 3-5, EC50 in the range of 1.27 to 1.58 mg/mL) as relevant sources of antiprotozoal substances. Furthermore, the pool measurements of ADP (adenosine diphosphate) and NTPs (nucleoside triphosphates) in the cultivated bloodstream form of trypanosomes exposed to different concentrations of EOs showed a disturbed energy metabolism as indicated by increased pools of ADP in comparison to ATP (adenosine triphosphate) and other NTPs. Ultimately, this study highlights the significant efficacy of A. nemorosa EO for developing long-lasting and effective antiprotozoal formulations, including nanoemulsions.

Antitrypanosomal activity of Anthriscus nemorosa essential oils and combinations of their main constituents

Cecilia Baldassarri
Primo
;
Giulia Falappa
Secondo
;
Eugenia Mazzara;Laura Acquaticci;Elena Ossoli;Diego Romano Perinelli;Giulia Bonacucina;Loredana Cappellacci;Filippo Maggi;Riccardo Petrelli
Ultimo
2021

Abstract

This study aimed to investigate the susceptibility of Trypanosoma brucei to the Anthriscus nemorosa essential oils (EOs), isolated compounds from these oils, and artificial mixtures of the isolated compounds in their conventional and nanoencapsulated forms. The chemical composition of the essential oils from the aerial parts and roots of Anthriscus nemorosa, obtained from a wild population growing in central Italy, were analyzed by gas chromatography/mass spectrometry(GC/MS). In both cases, the predominant class of compounds was monoterpene hydrocarbons, which were more abundant in the EOs from the roots (81.5%) than the aerial parts (74.0%). The overall results of this work have shed light on the biological properties of A. nemorosa EO from aerial parts (EC50 =1.17 mg/mL), farnesene (EC50 = 0.84 mg/mL) and artificial mixtures (Mix 3-5, EC50 in the range of 1.27 to 1.58 mg/mL) as relevant sources of antiprotozoal substances. Furthermore, the pool measurements of ADP (adenosine diphosphate) and NTPs (nucleoside triphosphates) in the cultivated bloodstream form of trypanosomes exposed to different concentrations of EOs showed a disturbed energy metabolism as indicated by increased pools of ADP in comparison to ATP (adenosine triphosphate) and other NTPs. Ultimately, this study highlights the significant efficacy of A. nemorosa EO for developing long-lasting and effective antiprotozoal formulations, including nanoemulsions.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11581/455012
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