Identification and quantification of natural compounds from medicinal and nutritional plants, biological fluids and cell extracts using GC-MS/MS

GC-MS/MS is a very efficient, robust, sensitive and cost effective analytical method to analyze small molecules directly or after derivatization. Its characteristics make it a useful complementary method to LC-MS. This equipment will be used to identify and quantify different natural compounds from plants, biological fluids and cellular extracts from the three co-promoters’ teams but also other collaborating laboratories. It will be particularly useful to support research projects focusing on the health-promoting effects (curative or preventive) of the following compounds: (1) antimicrobial and antiparasitic compounds from medicinal plant extracts, (2) lipid mediators involved in inflammatory diseases and immunity, (3) short chain fatty acids in multiple contexts as metabolism, cancer and microbiota characterization, (4) polyunsaturated fatty acids, conjugated or not, present in neglected plant sources from the international plant biodiversity and bearing potential anti-tumor properties, (5) potentially cytotoxic metabolites formed from these fatty acids by cellular and animal models of tumor development.