A high-efficiency sensing system for α-synuclein (α-Syn) oligomers was designed based on a novel one dimensional (1D)/2D structural nanohybrid (denoted as CoMnZIF@CNF) of CoMn-based zeolitic imidazolate framework nanosheets (CoMnZIF NSs) vertically grown around carbon nanofibers (CNFs). CNFs were prepared by calcining electrospun polyacrylonitrile under Ar/H2 atmosphere and used as the template for CoMnZIF synthesis, which can remarkably enlarge the electrochemical signal of the CoMnZIF@CNF nanohybrid. The series of CoMnZIF@CNF nanohybrids were modulated by changing the ratios of precursors Co(NO3)2 and Mn(NO3)2 (i.e. 3:1, 1:1, and 1:3), leading to optimized sensing performances of α-Syn oligomers. Owing to the hierarchical nanostructure, good biocompatibility, and strong bioaffinity, the α-Syn oligomer aptamer can be strongly anchored over the CoMnZIF@CNF nanohybrid. Although the three CoMnZIF@CNF nanohybrids show comparable electrochemical activity, the CoMnZIF@CNF(1–3)-based aptasensor exhibits the superior sensing performance to other CoMnZIF@CNF nanohybrids and reported MOFs, giving a low detection limit of 0.87 fg mL−1 (45.7 fM) within the concentration of α-Syn oligomers rangefrom 1 fg mL−1 (52.6 fM) to 0.2 ng mL−1 (0.1 nM). The aptasensor based on CoMnZIF@CNF also has excellent selectivity, stability, reproducibility, and usability for the detection of α-Syn oligomersin human serum. The efficient strategy of structural hybridization can be used in designing different aptasensors and further extend the application range of ZIFs materials.

Structural hybridization of bimetallic zeolitic imidazolate framework (ZIF) nanosheets and carbon nanofibers for efficiently sensing α-synuclein oligomers

Chuanpan Guo;Fenghe Duan;Fabio Marchetti;
2020-01-01

Abstract

A high-efficiency sensing system for α-synuclein (α-Syn) oligomers was designed based on a novel one dimensional (1D)/2D structural nanohybrid (denoted as CoMnZIF@CNF) of CoMn-based zeolitic imidazolate framework nanosheets (CoMnZIF NSs) vertically grown around carbon nanofibers (CNFs). CNFs were prepared by calcining electrospun polyacrylonitrile under Ar/H2 atmosphere and used as the template for CoMnZIF synthesis, which can remarkably enlarge the electrochemical signal of the CoMnZIF@CNF nanohybrid. The series of CoMnZIF@CNF nanohybrids were modulated by changing the ratios of precursors Co(NO3)2 and Mn(NO3)2 (i.e. 3:1, 1:1, and 1:3), leading to optimized sensing performances of α-Syn oligomers. Owing to the hierarchical nanostructure, good biocompatibility, and strong bioaffinity, the α-Syn oligomer aptamer can be strongly anchored over the CoMnZIF@CNF nanohybrid. Although the three CoMnZIF@CNF nanohybrids show comparable electrochemical activity, the CoMnZIF@CNF(1–3)-based aptasensor exhibits the superior sensing performance to other CoMnZIF@CNF nanohybrids and reported MOFs, giving a low detection limit of 0.87 fg mL−1 (45.7 fM) within the concentration of α-Syn oligomers rangefrom 1 fg mL−1 (52.6 fM) to 0.2 ng mL−1 (0.1 nM). The aptasensor based on CoMnZIF@CNF also has excellent selectivity, stability, reproducibility, and usability for the detection of α-Syn oligomersin human serum. The efficient strategy of structural hybridization can be used in designing different aptasensors and further extend the application range of ZIFs materials.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/440691
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