Worldwide, billions of people are at risk from viruses, parasites and bacteria transmitted by mosquitoes, ticks, fleas and other vectors. Over exploitation of chemical pesticides to overcome the mosquito borne diseases led to detrimental effects on environment and human health. The present research aims to explore bio-fabrication of metal silver nanomaterials using Rhazya stricta extract against deadly mosquito vectors and microbial pathogens. The phytochemical profile of the R. stricta extracts was studied by HPLC-MS and 1H NMR. Further, confirmation of the bio-fabricated silver nanoparticles (AgNPs) was car- ried out by UV–vis spectroscopy and characterization through FTIR, TEM, EDX, and XRD analyses. The R. stricta-fabricated AgNPs showed acute toxicity on key mosquito vectors from two different country (India and Kingdom of Saudi Arabia, KSA) strains, notably, with LC50 values of 10.57, 11.89 and 12.78 lg/ml on malarial, dengue and filarial key Indian strains of mosquito vectors, respectively, and 30.66 and 38.39 lg/ml on KSA strains of Aedes aegypti and Culex pipiens, respectively. In mosquito adulticidal activity, R. stricta extract alone exhibited LC50 values ranging from 304.34 to 382.45 lg/ml against Indian strains and from 738.733 to 886.886 against Saudi Arabian strains, while AgNPs LC50 boosted from 9.52 to 12.16 lg/ml and from 30.66 to 38.39 lg/ml, respectively. Moreover, it was noticed that at low concentra- tion the tested AgNPs showed high growth retardation of important pathogenic bacteria such as Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi with inhibition zone diameters from 11.86 to 22.92 mm. In conclusion, the present study highlighted that R. stricta-fabricated AgNPs could be a lead material for the management of mosquito vector and microbial pathogens control.
|Titolo:||Phytochemical analysis of Rhazya stricta extract and its use in fabrication of silver nanoparticles effective against mosquito vectors and microbial pathogens|
|Data di pubblicazione:||2020|
|Appare nelle tipologie:||Articolo|
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