Introduction: Adipokines have a role as a link between the energy availability and correct reproductive activity. Apelin (APLN) increases body weight but also lead to infertility modifying reproductive hormones in mouse. Expression of APLN and its receptor (APLNR) was observed in different tract of female genital system with differences among species. In this work, the expression and localization of APLN and APLNR were analyzed in the fallopian tubes of sheep. Materials and Methods: 15 Comisana x Appenninica adult female sheep in dry stage were fed with fresh hay from June to the pasture maximum flowering (MxF). From this period to maximum dryness, the control group (Cnt) was fed with fresh hay while, the experimental group (Exp) with fresh hay supplemented with 600g/day/head of barely and corn (1:1). Fallopian tubes were processed to perform RT-PCR and immunohistochemistry for APLN and APLNR. Results: RT-PCR evidenced the transcripts for both molecules. IHC showed APLN and APLNR staining in the epithelial cells of both the infundibulum and isthmus. APLN appeared stronger in Cnt and Exp groups respect to MxF. Conclusion: No studies describe APLN or its receptor in the fallopian tubes. Their identification in the sheep suggests that APLN plays a role in the salpinx activity and is involved in the reproduction. This is a preliminary report that introduces APLN investigation in the sheep female genital system however, the exact role of APLN and the influence of diet needs further elucidation.

Apelin expression in the fallopian tubes of ewes subjected to different nutritional levels: preliminary results

Paola Scocco;
2018

Abstract

Introduction: Adipokines have a role as a link between the energy availability and correct reproductive activity. Apelin (APLN) increases body weight but also lead to infertility modifying reproductive hormones in mouse. Expression of APLN and its receptor (APLNR) was observed in different tract of female genital system with differences among species. In this work, the expression and localization of APLN and APLNR were analyzed in the fallopian tubes of sheep. Materials and Methods: 15 Comisana x Appenninica adult female sheep in dry stage were fed with fresh hay from June to the pasture maximum flowering (MxF). From this period to maximum dryness, the control group (Cnt) was fed with fresh hay while, the experimental group (Exp) with fresh hay supplemented with 600g/day/head of barely and corn (1:1). Fallopian tubes were processed to perform RT-PCR and immunohistochemistry for APLN and APLNR. Results: RT-PCR evidenced the transcripts for both molecules. IHC showed APLN and APLNR staining in the epithelial cells of both the infundibulum and isthmus. APLN appeared stronger in Cnt and Exp groups respect to MxF. Conclusion: No studies describe APLN or its receptor in the fallopian tubes. Their identification in the sheep suggests that APLN plays a role in the salpinx activity and is involved in the reproduction. This is a preliminary report that introduces APLN investigation in the sheep female genital system however, the exact role of APLN and the influence of diet needs further elucidation.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11581/417400
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