Introduction: Hypericum androsaemum L. grows spontaneously in the Mediterranean area. Besides its employment as ornamental plant, H. androsaemum has also been used in folk medicine to treat depression. To better understand the beneficial role of this plant in mood disorders, the effect of H. androsaemum red berries water extract (HAWE) was studied in a mouse model of post-stroke depression (PSD). Methods: HAWE was characterized by HPLC-DAD analysis. Behavioural tests were carried out using a mice model of PSD. Two doses of HAWE were administered intraperitoneally. After that, mice were sacrificed and brain tissue was collected, in order to evaluate the in vivo antioxidant activity by measuring CAT, GSH, and SOD activity. TARS levels, markers of lipids peroxidation, were evaluated on serum samples. Results: The HPLC-DAD analysis showed that HAWE contained significant amounts of shikimic acid (110.0 g/kg), chlorogenic acid (56.9 g/kg), catechin (5.8 g/kg) and hyperoside (2.7 g/kg). Behavioural tests showed that WE has an antidepressive-like effect, reducing significantly the depression symptoms at its highest dosage and restoring normal behaviour. Furthermore, the in vivo analysis revealed that WE improves the oxidative stress by increasing endogenous antioxidant defences in brain tissue and ameliorating TBARS levels. Conclusion: This research work demonstrated the in vivo protective effects of HAWE in PSD for the first time. The results from behavioural tests were correlated with: 1) the antioxidant capacity, measured evaluating the antioxidant enzymes activity and TBARS levels, and 2) the characterization in the bioactive constituents of HAWE. Further studies are needed to highlight the mechanism of action of HAWE [1]. References: 1. Nabavi S.M., Nabavi S.F., Sureda A., Caprioli G., Iannarellic R., Tsetegho Sokengd A.J., Braidye N., Khanjanif S., Hajizadeh A., Moghaddam A.H., Atanasov A., Daglia M., Maggi F. The water extract of tutsan (Hypericum androsaemum L.) red berries exerts antidepressive-like effects and in vivo antioxidant activity in a mouse model of post-stroke depression. Biomedicine & Pharmacotherapy. 2018. 99: 290-298.
The antidepressive-like effect of water extract of Hypericum androsaemum L. red berries in a mouse model of post-stroke depression.
Giovanni Caprioli;Romilde Iannarelli;Filippo Maggi.
2018-01-01
Abstract
Introduction: Hypericum androsaemum L. grows spontaneously in the Mediterranean area. Besides its employment as ornamental plant, H. androsaemum has also been used in folk medicine to treat depression. To better understand the beneficial role of this plant in mood disorders, the effect of H. androsaemum red berries water extract (HAWE) was studied in a mouse model of post-stroke depression (PSD). Methods: HAWE was characterized by HPLC-DAD analysis. Behavioural tests were carried out using a mice model of PSD. Two doses of HAWE were administered intraperitoneally. After that, mice were sacrificed and brain tissue was collected, in order to evaluate the in vivo antioxidant activity by measuring CAT, GSH, and SOD activity. TARS levels, markers of lipids peroxidation, were evaluated on serum samples. Results: The HPLC-DAD analysis showed that HAWE contained significant amounts of shikimic acid (110.0 g/kg), chlorogenic acid (56.9 g/kg), catechin (5.8 g/kg) and hyperoside (2.7 g/kg). Behavioural tests showed that WE has an antidepressive-like effect, reducing significantly the depression symptoms at its highest dosage and restoring normal behaviour. Furthermore, the in vivo analysis revealed that WE improves the oxidative stress by increasing endogenous antioxidant defences in brain tissue and ameliorating TBARS levels. Conclusion: This research work demonstrated the in vivo protective effects of HAWE in PSD for the first time. The results from behavioural tests were correlated with: 1) the antioxidant capacity, measured evaluating the antioxidant enzymes activity and TBARS levels, and 2) the characterization in the bioactive constituents of HAWE. Further studies are needed to highlight the mechanism of action of HAWE [1]. References: 1. Nabavi S.M., Nabavi S.F., Sureda A., Caprioli G., Iannarellic R., Tsetegho Sokengd A.J., Braidye N., Khanjanif S., Hajizadeh A., Moghaddam A.H., Atanasov A., Daglia M., Maggi F. The water extract of tutsan (Hypericum androsaemum L.) red berries exerts antidepressive-like effects and in vivo antioxidant activity in a mouse model of post-stroke depression. Biomedicine & Pharmacotherapy. 2018. 99: 290-298.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
