The knowledge of the fungal mycobiota of arthropods, including the vectors of human and animal diseases, is still limited. Here, we investigated the mycobiota associated with the sand fly Phlebotomus perniciosus, the main vector of leishmaniasis in the western Mediterranean area, by a culture-dependent approach (microbiological analyses and sequencing of the 26S rRNA gene), internal transcribed spacer (ITS) rRNA amplicon-based next-generation sequencing, fluorescence in situ hybridisation (FISH), and genome sequencing of the dominant yeast species. The dominant species was Meyerozyma guilliermondii, known for its biotechnological applications. We focused the attention on this yeast and we investigated its prevalence in adults, pupae and larvae of reared sand flies (overall prevalence: 57.5%) and of field-collected individuals (overall prevalence: 9%). Using whole-mount FISH and microscopic examination, we further showed that M. guilliermondii colonizes the midgut of females, males and larvae and the distal part of Malpighian tubules of female sand flies, suggesting a possible role in urate degradation. Finally, the sequencing and analysis of the genome of M. guilliermondii allowed to predict the complete uric acid degradation pathway, suggesting that the yeast could contribute to the removal of the excess of nitrogenous wastes after the blood meal of the insect host. This article is protected by copyright. All rights reserved.

The mycobiota of the sand fly Phlebotomus perniciosus: involvement of yeast symbionts in uric acid metabolism

DE MARCO, LEONE;MENSAH, PRISCILLA;Ricci, Irene;GRADONI, LUIGI;BANDI, Claudio;Epis, Sara
2018-01-01

Abstract

The knowledge of the fungal mycobiota of arthropods, including the vectors of human and animal diseases, is still limited. Here, we investigated the mycobiota associated with the sand fly Phlebotomus perniciosus, the main vector of leishmaniasis in the western Mediterranean area, by a culture-dependent approach (microbiological analyses and sequencing of the 26S rRNA gene), internal transcribed spacer (ITS) rRNA amplicon-based next-generation sequencing, fluorescence in situ hybridisation (FISH), and genome sequencing of the dominant yeast species. The dominant species was Meyerozyma guilliermondii, known for its biotechnological applications. We focused the attention on this yeast and we investigated its prevalence in adults, pupae and larvae of reared sand flies (overall prevalence: 57.5%) and of field-collected individuals (overall prevalence: 9%). Using whole-mount FISH and microscopic examination, we further showed that M. guilliermondii colonizes the midgut of females, males and larvae and the distal part of Malpighian tubules of female sand flies, suggesting a possible role in urate degradation. Finally, the sequencing and analysis of the genome of M. guilliermondii allowed to predict the complete uric acid degradation pathway, suggesting that the yeast could contribute to the removal of the excess of nitrogenous wastes after the blood meal of the insect host. This article is protected by copyright. All rights reserved.
2018
File in questo prodotto:
File Dimensione Formato  
45.Martin_et_al-2018-Environmental_Microbiology.pdf

accesso aperto

Tipologia: Documento in Post-print
Licenza: DRM non definito
Dimensione 6.88 MB
Formato Adobe PDF
6.88 MB Adobe PDF Visualizza/Apri
Environmental Microbiology, 2018 vol. 20 pp. 1064–1077 - MARTIN.pdf

solo gestori di archivio

Tipologia: Versione Editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 516.94 kB
Formato Adobe PDF
516.94 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/406241
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 12
  • ???jsp.display-item.citation.isi??? 11
social impact