Ferulago campestris (Besser) Grecescu (Apiaceae), a perennial herb, has been traditionally used for the treatment of intestinal worms, haemorrhoids, ulcers, snake bites, as well as headache and diseases of the spleen. Here, we distilled the essential oil of F. campestris (OFC) and investigated its protective effect on D-galactosamine /lipopolysacchride (GalN/LPS) induced liver injury in rats. The major fraction of OFC was given by monoterpene hydrocarbons (78.8%) such as myrcene (33.4%) α-pinene (23.0%) and γ-terpinene (10.9%). Oral administration of OFC 20 and 50 mg/kg dramatically inhibited GalN/LPS-induced serum elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and malondialdehyde (MDA), and significantly ameliorated liver injury as evidence in H&E staining. Furthermore, real-time PCR assay showed that OFC treatment significantly inhibited GalN/LPS-induced mRNA expression of IL-1β, IL-6, and inducible nitric oxide synthase (iNOS) in liver tissues. In addition, western blotting assay showed that OFC treatment significantly reversed GalN/LPS-induced protein expression of cleaved caspase-3 and -7, Bcl-2 and Bcl-xL in liver tissues. In conclusion, this study showed that OFC protect GalN/LPS-induced liver injury in rats.
|Titolo:||The Chemical Constituents and the Hepatoprotective Effect of the Essential Oil Isolated from Ferulago campestris (Besser) Grecescu (Apiaceae) Fruits|
|Data di pubblicazione:||2016|
|Appare nelle tipologie:||Articolo|