Introduction: Platelet-derived growth factor A (PDGF-A) is produced by keratinocytes and expressed by epidermis in humans. It is implicated in the hair follicle (HF) morphogenesis and is supposed to be a positive regulator of the hair growth (Tomita et al., J Dermatol Sci 2006; 43(2):105-153). PDGF-A also acts as a mitogen in the cutaneous wound healings. In this study, PDGF-A and its receptor (PDGFR-A) were investigated in the skin of the dog by immunohistochemistry. Materials and Methods: A mouse monoclonal anti PDGF-A antibody and a rabbit polyclonal anti PDGFR-A antibody (Santa Cruz Biotechnology) were used. Skin specimens were collected from three fetuses, obtained from abortions, and five adult animals. The immunological reaction was detected with the ABC kit (Vector Laboratories) and visualized with diaminobenzidine. Results: PDGF-A and PDGFR-A were both identified in the developing skin of the fetuses as well as in the adults. In the fetuses, immunostaining was localized in the epithelium lining the body and in the epithelial buds growing into the dermis. In the adults, PDGF-A was observed in some structures of the skin including epidermis, HFs, blood vessels, arrector pili muscles and adipose tissue. In particular, positive cells were localized in the basal layer of the epidermis and the outer root sheath of the HFs. Likewise, PDGFR-A was observed in the epidermis and HFs; in both cases, staining was mainly localized in the suprabasal layers of epithelium. The receptor was also identified in the dermal sheath and in the dermal papilla of the HFs. Conclusion: This study suggests an involvement of PDGF-A in the biology of the skin in the canine species. PDGF-A and its receptor were identified in close apposition both in the epidermis and HFs suggesting a paracrine and/or autocrine action of this molecule during skin morphogenesis and hair cycle.
Expression of Platelet-derived growth factor-A and its receptor in the skin of the dog.
PAZZAGLIA, IRENE;SCOCCO, Paola;
2016-01-01
Abstract
Introduction: Platelet-derived growth factor A (PDGF-A) is produced by keratinocytes and expressed by epidermis in humans. It is implicated in the hair follicle (HF) morphogenesis and is supposed to be a positive regulator of the hair growth (Tomita et al., J Dermatol Sci 2006; 43(2):105-153). PDGF-A also acts as a mitogen in the cutaneous wound healings. In this study, PDGF-A and its receptor (PDGFR-A) were investigated in the skin of the dog by immunohistochemistry. Materials and Methods: A mouse monoclonal anti PDGF-A antibody and a rabbit polyclonal anti PDGFR-A antibody (Santa Cruz Biotechnology) were used. Skin specimens were collected from three fetuses, obtained from abortions, and five adult animals. The immunological reaction was detected with the ABC kit (Vector Laboratories) and visualized with diaminobenzidine. Results: PDGF-A and PDGFR-A were both identified in the developing skin of the fetuses as well as in the adults. In the fetuses, immunostaining was localized in the epithelium lining the body and in the epithelial buds growing into the dermis. In the adults, PDGF-A was observed in some structures of the skin including epidermis, HFs, blood vessels, arrector pili muscles and adipose tissue. In particular, positive cells were localized in the basal layer of the epidermis and the outer root sheath of the HFs. Likewise, PDGFR-A was observed in the epidermis and HFs; in both cases, staining was mainly localized in the suprabasal layers of epithelium. The receptor was also identified in the dermal sheath and in the dermal papilla of the HFs. Conclusion: This study suggests an involvement of PDGF-A in the biology of the skin in the canine species. PDGF-A and its receptor were identified in close apposition both in the epidermis and HFs suggesting a paracrine and/or autocrine action of this molecule during skin morphogenesis and hair cycle.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
