Alpha-lipoic acid (ALA) plays a crucial role as antioxidant and metabolic component of some enzymatic complexes involved in glucose metabolism of different cell types. In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme-(ALA). GFAP expression significantly increased after (R+)enantiomer acute-treatment and also in glutamate-pretreated ones. R(+)enantiomer acutetreatment increased vimentin expression, but it decreased after raceme acute-treatment. Nestin expression drastically increased after acute raceme-treatment in glutamate-pretreated or unpretreated cultures, but significantly decreased after (R+)enantiomer acute and chronic-treatments. Cyclin D1 expression much increased in raceme acute-treated astrocyte cultures pretreated with glutamate. MAP-kinase expression slightly increased after (R+)enantiomer acute treatment in glutamate-pretreated or unpretreated ones. Immunocyto - chemical analysis is well correlated with Western blot and ELISA data. Our results indicate a significant increase of GFAP expression as well as an “up and down” modulation of nestin and vimentin expression in 15 DIV astrocyte cultures after chronic or acute treatment with raceme or (R+)enantiomer ALA. These preliminary findings may better clarify antioxidant and metabolic role played by ALA in proliferating and differentiating astrocyte cultures suggesting an interactive cross-talk between glial and neuronal cells, after brain lesions or damages.

IMMUNOCYTOCHEMICAL, WESTERN BLOT AND ELISA ANALYSIS EVALUATION OF ASTROGLIAL BIOMARKERS IN ASTROCYTE CULTURES TREATED WITH ALPHA-LIPOIC ACID

TOMASSONI, Daniele;AMENTA, Francesco;
2011-01-01

Abstract

Alpha-lipoic acid (ALA) plays a crucial role as antioxidant and metabolic component of some enzymatic complexes involved in glucose metabolism of different cell types. In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme-(ALA). GFAP expression significantly increased after (R+)enantiomer acute-treatment and also in glutamate-pretreated ones. R(+)enantiomer acutetreatment increased vimentin expression, but it decreased after raceme acute-treatment. Nestin expression drastically increased after acute raceme-treatment in glutamate-pretreated or unpretreated cultures, but significantly decreased after (R+)enantiomer acute and chronic-treatments. Cyclin D1 expression much increased in raceme acute-treated astrocyte cultures pretreated with glutamate. MAP-kinase expression slightly increased after (R+)enantiomer acute treatment in glutamate-pretreated or unpretreated ones. Immunocyto - chemical analysis is well correlated with Western blot and ELISA data. Our results indicate a significant increase of GFAP expression as well as an “up and down” modulation of nestin and vimentin expression in 15 DIV astrocyte cultures after chronic or acute treatment with raceme or (R+)enantiomer ALA. These preliminary findings may better clarify antioxidant and metabolic role played by ALA in proliferating and differentiating astrocyte cultures suggesting an interactive cross-talk between glial and neuronal cells, after brain lesions or damages.
2011
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/370020
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact