Leptin isapolypeptidesecretedbyadipocyteswhichbindstoaspecificreceptor(Ob-R)thatisexpressed in varioustissues.ThewidedistributionoftheOb-Rsuggeststhatleptinmightexertdiversebiological functions, notonlybyregulatingenergymetabolismandappetite,butalsobyactingasamitogenin many celltypes,includingkeratinocytes. In thisstudy,thepresenceandlocalizationofOb-RwasinvestigatedintheskinofthedogusingRT-PCR and immunohistochemicaltechniques.RT-PCRrevealedthepresenceofOb-Rm-RNAintheskinspeci- mens collectedfromthedorsalregionoftwosmoothcoatbreeddogs.Throughimmunohistochemistry performed ontheskinoffivedogs,theexpressionofthereceptorwasobservedinthebasallayerofthe epidermis, inthehairfolliclesaswellasintheapocrinesweatandsebaceousglands.NostainingforOb-R was detectedinthesuprabasalepidermislayers.Strongpositivesignalswereobservedinmanycellsof the outerrootsheathofhairfolliclesingrowingandinregressivephases.TheidentificationofOb-Rin the abovetargetssuggeststhatleptinmayplayaroleintheregulationofcyclicrenewaloftheepidermis and skinappendagesindog.Thisstudyrepresentsanimportantcontributiontounderstandthecomplex mechanisms thatareinvolvedintheskinbiologyinthisspecies.

Leptin receptor is expressed by epidermis and skin appendages in dog

SCOCCO, Paola;
2014-01-01

Abstract

Leptin isapolypeptidesecretedbyadipocyteswhichbindstoaspecificreceptor(Ob-R)thatisexpressed in varioustissues.ThewidedistributionoftheOb-Rsuggeststhatleptinmightexertdiversebiological functions, notonlybyregulatingenergymetabolismandappetite,butalsobyactingasamitogenin many celltypes,includingkeratinocytes. In thisstudy,thepresenceandlocalizationofOb-RwasinvestigatedintheskinofthedogusingRT-PCR and immunohistochemicaltechniques.RT-PCRrevealedthepresenceofOb-Rm-RNAintheskinspeci- mens collectedfromthedorsalregionoftwosmoothcoatbreeddogs.Throughimmunohistochemistry performed ontheskinoffivedogs,theexpressionofthereceptorwasobservedinthebasallayerofthe epidermis, inthehairfolliclesaswellasintheapocrinesweatandsebaceousglands.NostainingforOb-R was detectedinthesuprabasalepidermislayers.Strongpositivesignalswereobservedinmanycellsof the outerrootsheathofhairfolliclesingrowingandinregressivephases.TheidentificationofOb-Rin the abovetargetssuggeststhatleptinmayplayaroleintheregulationofcyclicrenewaloftheepidermis and skinappendagesindog.Thisstudyrepresentsanimportantcontributiontounderstandthecomplex mechanisms thatareinvolvedintheskinbiologyinthisspecies.
2014
262
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/358183
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