Neutral and charged phosphine/scorpionate copper(I) complexes: Effects of ligand assembly on their antiproliferative activity

Ligand-exchange reactions of copper(I) precursors ([Cu(CH3CN)4]BF4, CuCl) with a panel of bis(azolyl)borates or poly(pyrazolyl)methanes and a tertiary monodentate phosphine (PTA ¼ 1,3,5-triaza-7-phosphaadamantane, PCN ¼ tris(cyanoethyl)phosphine) produced two series of heteroleptic, either ‘2 þ 1 þ 1’- or ‘3 þ 1’-type complexes, which have been characterized by elemental analysis, FT-IR, ESIMS and multinuclear 31P and 1H NMR. ‘2 þ 1 þ 1’-type complexes include a N,N-bidentate chelate and two monodentate phosphines (1e8) and ‘3 þ 1’-type complexes comprise a N,N,O- or N,N,N-tridentate chelate and one monodentate phosphine (9e12). All these complexes adopt a four-coordinate, tetrahedral geometry. ‘3 þ 1’ complexes show better red-ox stability and a greater tendency to retain the native ‘3 þ 1’ mixed-ligand structure. Conversely, ‘2 þ 1 þ 1’ complexes exhibit increased propensity to dissociation as shown by ESI-MS measurements and X-ray structure determination at low temperature (150 K) of the polymeric complex {[H2B(tzNO2)2]Cu[PCN]}n 6b. In this complex, either the bis(triazolyl)borate and the PCN ligands act as bidentate, with PCN being also the m2ebridiging linker between adjacent monomers. Compound 6b is the first reported example of a polymeric PCN compound with a tetra-coordinate metal centre. Cytotoxic activity of all compounds has been evaluated by MTT test against a panel of several human tumor cell lines including examples of breast (MCF-7), colon (HCT-15 and LoVo), lung (A549), cervix (A431) and ovarian (2008 and its cisplatin resistant variant, C13*) carcinoma, melanoma (A375) and promyelocytic leukemia (HL60). Copper complexes generally show in vitro antitumour activity comparable to that of cisplatin. In particular, neutral ‘3 þ 1’-type complexes 9 and 10, show IC50 values appreciably lower than those exhibited by the reference metallodrug.