We used a combination of radioreceptor binding and autoradiographic techniques to study the pharmacological characteristics and anatomical localization of [3H]quinuclidinyl benzylate (QNB) in kidney sections of Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). [3H]QNB was bound by sections of rat kidney in a manner consistent with the labeling of muscarinic acetylcholine receptors. Anatomically, these receptor sites were located primarily within the smooth muscle of the renal vascular tree and to a lesser extent within cortical and medullary tubules. The density of renal muscarinic acetylcholine receptors (both vascular and tubular) was decreased in hypertension but the affinity of the ligand for muscarinic receptors was unchanged. A possible role of muscarinic acetylcholine receptors in renal function is suggested.

Autoradiographic localization of muscarinic receptors within the rat kidney.

AMENTA, Francesco;
1989

Abstract

We used a combination of radioreceptor binding and autoradiographic techniques to study the pharmacological characteristics and anatomical localization of [3H]quinuclidinyl benzylate (QNB) in kidney sections of Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). [3H]QNB was bound by sections of rat kidney in a manner consistent with the labeling of muscarinic acetylcholine receptors. Anatomically, these receptor sites were located primarily within the smooth muscle of the renal vascular tree and to a lesser extent within cortical and medullary tubules. The density of renal muscarinic acetylcholine receptors (both vascular and tubular) was decreased in hypertension but the affinity of the ligand for muscarinic receptors was unchanged. A possible role of muscarinic acetylcholine receptors in renal function is suggested.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11581/242984
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact