We investigated the lectin cytochemistry of control and sialidase-digested sections of the mouse parotid gland by postembedding techniques. PNA and DBA lectins were used and their affinity sites were localized by employing conjugates with horseradish peroxidase that then reacted with anti-horseradish peroxidase antibody and protein A-gold. Potassium hydroxide pretreatment also was used before sialidase/PNA and DBA binding to investigate sialic acid acetylation. Ultrathin sections were obtained from specimens embedded in the acrylic hydrophilic resin, Bioacryl. The acini of mouse parotid gland contained polymorphous secretory grannies differentially stained by the two lectins; the use of sialidase digestion and KOH deacetylation revealed that the sialic acids linked to beta-galactose are restricted to the electron-dense concentric areas of target granules, whereas the sialic acids linked to alpha-N-acetylgalactosamine contain C-4-acetylated groups and are preferentially located in rite electron-lucent regions of bizonal granules

Sialylation patterns of the mouse parotid secretory granules. Combined deacetylation, enzymatic degradation and lectin-gold binding / Menghi G; Marchetti L; Bondi AM. - In: EUROPEAN JOURNAL OF MORPHOLOGY. - ISSN 0924-3860. - 34:3(1995), pp. 181-185.

Sialylation patterns of the mouse parotid secretory granules. Combined deacetylation, enzymatic degradation and lectin-gold binding

MARCHETTI, Luigi;
1995

Abstract

We investigated the lectin cytochemistry of control and sialidase-digested sections of the mouse parotid gland by postembedding techniques. PNA and DBA lectins were used and their affinity sites were localized by employing conjugates with horseradish peroxidase that then reacted with anti-horseradish peroxidase antibody and protein A-gold. Potassium hydroxide pretreatment also was used before sialidase/PNA and DBA binding to investigate sialic acid acetylation. Ultrathin sections were obtained from specimens embedded in the acrylic hydrophilic resin, Bioacryl. The acini of mouse parotid gland contained polymorphous secretory grannies differentially stained by the two lectins; the use of sialidase digestion and KOH deacetylation revealed that the sialic acids linked to beta-galactose are restricted to the electron-dense concentric areas of target granules, whereas the sialic acids linked to alpha-N-acetylgalactosamine contain C-4-acetylated groups and are preferentially located in rite electron-lucent regions of bizonal granules
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11581/242114
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