This study is focused on a new approach for the determination of Leu-enkephalin loading efficiency in poly(isobutylcyanoacrylate)nanoparticles coated with depolymerized and thiolated chitosan (Mw 30, 85 and 145 kDa), Nanoparticles were obtained by anionic emulsion polymerization. The aim of this work was to propose a reliable method for the determination of the loading efficiency of Leu-enkephalin in nanoparticles. The amount of Leu-enkephalin contained in nanoparticles was determined after separation of loaded Leu-enkephalin from the dispersing medium using a modern and fast ultrafiltration technique as alternative to ultracentrifugation. The amount of Leu-enkephalin entrapped in nanoparticles was determined after total dissolution of nanoparticles in DMSO. Finally, the method developed in the present work was applied to the determination of the loading efficiency of nanoparticles prepared by two methods. The method consisting in loading nanoparticles with Leu-enkephalin at the same time as the preparation of the nanoparticles yielded higher loading efficiency (from 85 to 92%) than the method based on the adsorption of Leu-enkephalin on preformed nanoparticles (from 62 to 65%).

A new approach to determine loading efficiency of Leu-enkephalin in poly(isobutylcyanoacrylate) nanoparticles coated with thiolated chitosan

PALMIERI, Giovanni Filippo;
2008

Abstract

This study is focused on a new approach for the determination of Leu-enkephalin loading efficiency in poly(isobutylcyanoacrylate)nanoparticles coated with depolymerized and thiolated chitosan (Mw 30, 85 and 145 kDa), Nanoparticles were obtained by anionic emulsion polymerization. The aim of this work was to propose a reliable method for the determination of the loading efficiency of Leu-enkephalin in nanoparticles. The amount of Leu-enkephalin contained in nanoparticles was determined after separation of loaded Leu-enkephalin from the dispersing medium using a modern and fast ultrafiltration technique as alternative to ultracentrifugation. The amount of Leu-enkephalin entrapped in nanoparticles was determined after total dissolution of nanoparticles in DMSO. Finally, the method developed in the present work was applied to the determination of the loading efficiency of nanoparticles prepared by two methods. The method consisting in loading nanoparticles with Leu-enkephalin at the same time as the preparation of the nanoparticles yielded higher loading efficiency (from 85 to 92%) than the method based on the adsorption of Leu-enkephalin on preformed nanoparticles (from 62 to 65%).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11581/233274
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