Prostaglandin F2alpha (PGF2alpha), prostaglandin E2 (PGE2), progesterone, androgens, and estradiol-17beta in vitro basal release by follicles of the oviparous lizard, Podarcis s. sicula was studied; in addition, the in vitro effect of PGF2alpha and PGE2 on sex steroid release was evaluated. Follicles were divided according to the different vitellogenic developmental stages: pre-vitellogenic, early-vitellogenic, mid-vitellogenic and fully-grown. PGF2alpha and progesterone basal release was highest in fully-grown follicles; PGE2 and estradiol basal release was highest in early-vitellogenic follicles; androgens basal release was detectable in mid-vitellogenic and fully-grown vitellogenic follicles only. PGF2alpha increased progesterone release by fully-grown follicles; PGE2 increased estradiol release by all follicle types, except by early-vitellogenic ones. The present data suggest that PGF2alpha and PGE2 exert different roles on follicles: PGF2alpha seems to induce ovulation through the mediation of progesterone, while PGE2 seems to be implied in the start and the sustaining of oocyte vitellogenic development through the mediation of estradiol.
PROSTAGLANDINS AND SEX STEROIDS FROM REPTILIAN (PODARCIS-SICULA-SICULA) OVARIAN FOLLICLES AT DIFFERENT DEVELOPMENTAL STAGES
GOBBETTI, Anna;ZERANI, Massimo;
1993-01-01
Abstract
Prostaglandin F2alpha (PGF2alpha), prostaglandin E2 (PGE2), progesterone, androgens, and estradiol-17beta in vitro basal release by follicles of the oviparous lizard, Podarcis s. sicula was studied; in addition, the in vitro effect of PGF2alpha and PGE2 on sex steroid release was evaluated. Follicles were divided according to the different vitellogenic developmental stages: pre-vitellogenic, early-vitellogenic, mid-vitellogenic and fully-grown. PGF2alpha and progesterone basal release was highest in fully-grown follicles; PGE2 and estradiol basal release was highest in early-vitellogenic follicles; androgens basal release was detectable in mid-vitellogenic and fully-grown vitellogenic follicles only. PGF2alpha increased progesterone release by fully-grown follicles; PGE2 increased estradiol release by all follicle types, except by early-vitellogenic ones. The present data suggest that PGF2alpha and PGE2 exert different roles on follicles: PGF2alpha seems to induce ovulation through the mediation of progesterone, while PGE2 seems to be implied in the start and the sustaining of oocyte vitellogenic development through the mediation of estradiol.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
