The objectives of these experiments were to found in alpaca (Lama pacos) testis the presence of GnRH receptor, as well as to study the effects of this decapeptide on the testicular endocrine activity. Testes of sexually mature alpacas were used for immunohistochemistry and in vitro studies. Testis pieces were incubated with analogue (buserelin) or antagonist (antide ) of GnRH. The levels of testosterone, PGF2alpha and PGE2 were determined by RIA. GnRH receptor was immunolocalized in the cytoplasm of Leydig cells. Buserelin increased testosterone, PGF2alpha and PGE2 in vitro secretion, while antide reduced the above hormone secretion. The present study indicates, for the first time, that GnRH is one of the main factors that directly modulate, by paracrine and/or autocrine mechanisms, the testicular activity of alpaca
GNRH and testis of alpaca (Lama pacos): occurence of receptor and in vitro effects
ZERANI, Massimo;PARILLO, Francesco;VULLO, CECILIA;CATONE, Giuseppe
2009-01-01
Abstract
The objectives of these experiments were to found in alpaca (Lama pacos) testis the presence of GnRH receptor, as well as to study the effects of this decapeptide on the testicular endocrine activity. Testes of sexually mature alpacas were used for immunohistochemistry and in vitro studies. Testis pieces were incubated with analogue (buserelin) or antagonist (antide ) of GnRH. The levels of testosterone, PGF2alpha and PGE2 were determined by RIA. GnRH receptor was immunolocalized in the cytoplasm of Leydig cells. Buserelin increased testosterone, PGF2alpha and PGE2 in vitro secretion, while antide reduced the above hormone secretion. The present study indicates, for the first time, that GnRH is one of the main factors that directly modulate, by paracrine and/or autocrine mechanisms, the testicular activity of alpacaI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
