Guttural pouch mycosis is an emergency disease of the upper respiratory tract in equine species. In the present report a case of guttural pouch mycosis in a female, seven year-old pregnant donkey is described. A serious dyspnea which necessitated tracheotomy and preceding epistaxis was the most important clinical feature of guttural pouch mycosis in the donkey. A full and rapid effectiveness of the topical therapy, the protocol for which is described, is the main distinguishing feature with regard to treatment. In the Authors’ knowledge a detailed description of clinical features, treatment and follow up of guttural pouch mycosis in a donkey is not available in the scientific literature. The anatomical and physiological peculiarity of donkeys