The regional distribution and relative occurrence of phthalates were studied immuno-histochemically by confocal laser scanning microscopy in the alimentary tract of the green frog, Rana esculenta, using an antibody against o-phthalate esters. Many positive sites indicating the basal presence of phthalate esters were identified. The immunoreactive cells were located in the gastric glands of the stomach and in the intestinal epithelium regions with variable frequencies. The regional distribution of phathalate-accumulating cells resembled that of fish and demonstrated that these endocrine disrupters not only enter via the alimentary canal, but also bioaccumulate inside cells specialized in secretion as well as absorption functions.
In situ visualization of o-phthalate esters in gastrointestinal tract of the frog Rana esculenta
MARCHETTI, Luigi;SABBIETI, Maria Giovanna;
2003-01-01
Abstract
The regional distribution and relative occurrence of phthalates were studied immuno-histochemically by confocal laser scanning microscopy in the alimentary tract of the green frog, Rana esculenta, using an antibody against o-phthalate esters. Many positive sites indicating the basal presence of phthalate esters were identified. The immunoreactive cells were located in the gastric glands of the stomach and in the intestinal epithelium regions with variable frequencies. The regional distribution of phathalate-accumulating cells resembled that of fish and demonstrated that these endocrine disrupters not only enter via the alimentary canal, but also bioaccumulate inside cells specialized in secretion as well as absorption functions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
