In vivo and in vitro comparison of the effects of FGF-2 null and haplo-insufficiency on bone formation in mice

Naganawa, T.; Xiao, L.; Abogunde, E.; Sobue, T.; Kalajzic, I.; Sabbieti, Maria Giovanna; Agas, Dimitrios; Hurley, M. M.

doi:10.1016/j.bbrc.2005.10.215

We previously reported that deletion of the Fgf2 gene (Fgf2-/-) resulted in decreased bone mass in adult mice. This study examines the effect of haplo-insuffiency (Fgf2+/-) on bone loss in vertebrae from these mutant mice. Fgf2+/+ mice attained peak bone mass at 8-9 months of age. In contrast BMD was significantly reduced in vertebrae from adult (8-9) Fgf2+/- mice. Exogenous FGF-2 rescued reduced bone nodule formation in Fgf2+/- and Fgf2-/- cultures. Runx2 mRNA was reduced in cultures from Fgf2+/- and Fgf2-/- mice. FGF receptor2 mRNA and protein were markedly reduced in Fgf2+/- and Fgf2-/- mice. Decreased bone formation in Fgf2 mutant mice may correlate with impaired FGFR signaling, decreased Runx2 gene expression.

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Titolo:	In vivo and in vitro comparison of the effects of FGF-2 null and haplo-insufficiency on bone formation in mice
Autori:	NAGANAWA T. XIAO L. ABOGUNDE E. SOBUE T. KALAJZIC I. SABBIETI, Maria Giovanna AGAS, DIMITRIOS HURLEY M. M. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2006
Rivista:	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Abstract:	We previously reported that deletion of the Fgf2 gene (Fgf2-/-) resulted in decreased bone mass in adult mice. This study examines the effect of haplo-insuffiency (Fgf2+/-) on bone loss in vertebrae from these mutant mice. Fgf2+/+ mice attained peak bone mass at 8-9 months of age. In contrast BMD was significantly reduced in vertebrae from adult (8-9) Fgf2+/- mice. Exogenous FGF-2 rescued reduced bone nodule formation in Fgf2+/- and Fgf2-/- cultures. Runx2 mRNA was reduced in cultures from Fgf2+/- and Fgf2-/- mice. FGF receptor2 mRNA and protein were markedly reduced in Fgf2+/- and Fgf2-/- mice. Decreased bone formation in Fgf2 mutant mice may correlate with impaired FGFR signaling, decreased Runx2 gene expression.
Handle:	http://hdl.handle.net/11581/201287
Appare nelle tipologie:	Articolo

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