The present investigation extends our previous studies on PGF2α-mediated signalling in osteoblast metabolism. In particular, the role of PGF2α as modulator of heparan sulphate proteoglycans (HSPGs), fibroblast growth factor 2 (FGF-2) and fibroblast growth factor receptors (FGFRs) was evaluated. We hereby reported the novel observation that PGF2α was able to promote the formation of HSPGs/FGF-2/FGFRs complexes. Moreover, our data suggested that PGF2α could induce new synthesis of heparan sulphate (HS) chains on osteoblasts by a mec