Among a set of wild-type strains of Euplotes nobilii, every one derived asexually from one specimen isolated from Terra Nova Bay (Ross Sea, Antarctica), two were found to be representative of different mating types mutually capable of inducing each other to form mating pairs through pheromones constitutively secreted into the extracellular environment. Pheromones of strain AC-1 were purified to homogeneity and shown to be represented by two distinct proteins, that were denoted En-1 and En-2. En-1, secreted in amounts three-fold greater than En-2, was determined to have a molecular weight of 5617 and an asparagine at the N-terminus of its amino acid sequence, while En-2 has a molecular weight of 6290 and bears an asparctic acid at its N-terminus. The fact that En-1 and En-2 are coreleased by genetically identical cells of the same strain was taken to imply that they carry a heterozygotic combination of allelic pheromone genes and that these genes are regulated by relationships of co-dominance.

Purification and initial characterization of two pheromones from the marine Antartic cilate, Euplotes nobilii

FELICI, Alberto;ALIMENTI, Claudio;ORTENZI, Claudio;LUPORINI, Pierangelo
1999-01-01

Abstract

Among a set of wild-type strains of Euplotes nobilii, every one derived asexually from one specimen isolated from Terra Nova Bay (Ross Sea, Antarctica), two were found to be representative of different mating types mutually capable of inducing each other to form mating pairs through pheromones constitutively secreted into the extracellular environment. Pheromones of strain AC-1 were purified to homogeneity and shown to be represented by two distinct proteins, that were denoted En-1 and En-2. En-1, secreted in amounts three-fold greater than En-2, was determined to have a molecular weight of 5617 and an asparagine at the N-terminus of its amino acid sequence, while En-2 has a molecular weight of 6290 and bears an asparctic acid at its N-terminus. The fact that En-1 and En-2 are coreleased by genetically identical cells of the same strain was taken to imply that they carry a heterozygotic combination of allelic pheromone genes and that these genes are regulated by relationships of co-dominance.
1999
262
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/116959
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 14
  • ???jsp.display-item.citation.isi??? 15
social impact