The stability constants (KML) of Cu(II), Co(II), Zn(II), Ni(II), and Mn(II) chelates with 3, 8, 13, 18 -tetrametyl-21H, 23H-porphine -2,7,12,17- tetrapropionic acid, or coproporphyrin-I (CPI) have been determined by reversed-phase high performance liquid chromatography (RP-HPLC) on a octadecyl-bonded silica gel (C18 silica) with developing solvent (69:31 v/v) methanol-buffer at pH 7 (buffer ionic strength 0.270 mol L−1: 0.018 mol L−1 boric, phosphoric and acetic acid, and 0.036 mol L−1 sodium hydroxide), and 0.050 mol L−1 of NaCl, with UV detection. The values of log KML were 6.02±0.26 for the Cu(II), 5.37±0.18 for the Co(II), 4.51± 0.21 for the Zn(II), 4.52± 0.21 for the Ni(II) and 6.38± 0.04 for the Mn(II).
Determination of stability constants of Cu(II), Co(II), Zn(II), Ni(II) and Mn(II) chelates with 3,8,13,18-tetramethyl-21H,23H-porphine-2,7,12,17-tetrapropionic acid by reversed-phase high performance liquid chromatography
GIOVANNETTI, Rita;BARTOCCI, Vito
1999-01-01
Abstract
The stability constants (KML) of Cu(II), Co(II), Zn(II), Ni(II), and Mn(II) chelates with 3, 8, 13, 18 -tetrametyl-21H, 23H-porphine -2,7,12,17- tetrapropionic acid, or coproporphyrin-I (CPI) have been determined by reversed-phase high performance liquid chromatography (RP-HPLC) on a octadecyl-bonded silica gel (C18 silica) with developing solvent (69:31 v/v) methanol-buffer at pH 7 (buffer ionic strength 0.270 mol L−1: 0.018 mol L−1 boric, phosphoric and acetic acid, and 0.036 mol L−1 sodium hydroxide), and 0.050 mol L−1 of NaCl, with UV detection. The values of log KML were 6.02±0.26 for the Cu(II), 5.37±0.18 for the Co(II), 4.51± 0.21 for the Zn(II), 4.52± 0.21 for the Ni(II) and 6.38± 0.04 for the Mn(II).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
