Contraction of rat epididymal vas deferens is regulated via a release of neurotransmitters from autonomic nerves and is mediated by α1-adrenoceptors. This study was directed to the characterization of α1- adrenoceptors involved in the contraction of the epididymal portion of young CD rat vas deferens, that were selectively discriminated in two populations through the irreversible blockade of two β-chloroethyamines, 1 and 2. The antagonist activity of known subtype-selective α1-adrenoceptor antagonists, WB4101, 5-MU, and RS17053 (α1A), (+)-cyclazosin (α1B), and BMY7378 (α1D), was evaluated in the α1-adrenoceptors of the studied tissue as such and after pre-treatment with a proper discriminating concentration ofβ-chloroetylamines 1 and 2, comparing the results with the affinities determined in classical Wistar rat models: prostatic vas deferens (α1A), spleen (α1B), and thoracic aorta (α1D). The results suggested that two α1A-adrenoceptors are involved in the contraction of the epididymalvas deferens of young CD rats. These may represent two α1A-adrenoceptor isoforms that are selectively and irreversibly blocked by β-chloroetylamines 1 and 2, and reversibly antagonized by RS17053. The minor population, preferentially blocked by 1, seems correspond to a classical α1A-adrenoceptor subtype, while the major population, preferentially blocked by 2 and antagonized by RS17053 with low affinity, seems to correspond to an α1L-adrenoceptor.

Identification of alpha(1)-adrenoceptor subtypes involved in contraction of young CD rat epididymal vas deferens

BUCCIONI, Michela;ANGELI, Piero;CRISTALLI, Gloria;DAL BEN, DIEGO;LAMBERTUCCI, Catia;VOLPINI, Rosaria;MARUCCI, Gabriella
2009-01-01

Abstract

Contraction of rat epididymal vas deferens is regulated via a release of neurotransmitters from autonomic nerves and is mediated by α1-adrenoceptors. This study was directed to the characterization of α1- adrenoceptors involved in the contraction of the epididymal portion of young CD rat vas deferens, that were selectively discriminated in two populations through the irreversible blockade of two β-chloroethyamines, 1 and 2. The antagonist activity of known subtype-selective α1-adrenoceptor antagonists, WB4101, 5-MU, and RS17053 (α1A), (+)-cyclazosin (α1B), and BMY7378 (α1D), was evaluated in the α1-adrenoceptors of the studied tissue as such and after pre-treatment with a proper discriminating concentration ofβ-chloroetylamines 1 and 2, comparing the results with the affinities determined in classical Wistar rat models: prostatic vas deferens (α1A), spleen (α1B), and thoracic aorta (α1D). The results suggested that two α1A-adrenoceptors are involved in the contraction of the epididymalvas deferens of young CD rats. These may represent two α1A-adrenoceptor isoforms that are selectively and irreversibly blocked by β-chloroetylamines 1 and 2, and reversibly antagonized by RS17053. The minor population, preferentially blocked by 1, seems correspond to a classical α1A-adrenoceptor subtype, while the major population, preferentially blocked by 2 and antagonized by RS17053 with low affinity, seems to correspond to an α1L-adrenoceptor.
2009
262
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/112288
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 9
social impact