Utilizza questo identificativo per citare o creare un link a questo documento:
|Titolo:||Monitoring a genetically engineered bacterium in a freshwater environment by rapid enzymatic amplification of a synthetic DNA "number plate".|
|Autori interni:||AMICI, Augusto|
|Data di pubblicazione:||1991|
|Rivista:||APPLIED MICROBIOLOGY AND BIOTECHNOLOGY|
|Abstract:||In order to set up a sensitive and reliable detection method to monitor environmentally releases genetically engineered microorganisms (GEMs) a 72-bp, double-stranded DNA fragment has been built by annealing and ligating four synthetic oligonucleotides. Binding sites for two 20-mer oligonucleotides are situated inside the DNA fragment, flanking the centre. Into the central part of the construction a 30-nucleotide identification sequence has been fitted. Thanks to the presence of the two oligonucleotide binding sites, the synthetic construction ('number-plate') can be submitted to enzymatic amplification using the polymerase chain reaction (PCR), thus enabling the identification system to take advantage of the outstanding sensitivity of this technique. When released into a freshwater microcosm, cells of Pseudomonas putida carrying a 'number-plated' chromosome could be easily and rapidly detected merely by submitting boiled cell sediments to PCR amplification.|
|Appare nelle tipologie:||Articolo|
File in questo prodotto:
Non ci sono file associati a questo prodotto.
- PubMed Central loading...
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.