OVERFEEDING IMPACT ON NEURONAL AND GLIAL RESPONSES IN NOTHOBRANCHIUS FURZERI

The interaction between nutrients and brain activity is crucial to understand the mechanisms underlying energy balance and metabolic regulation. The balance between caloric intake and energy expenditure is regulated by complex neuroendocrine mechanisms, including the mTORC1 signaling pathway, which responds to nutrient availability and regulates neuronal plasticity. Numerous studies suggest that dietary variations can modulate brain activity and metabolic signaling, influencing both satiety mechanisms and neuronal responses. However, the impact of different dietary regimens on these processes and their consequences on the aging phenotype remain underexplored. In this context, short- -lived models such as Nothobranchius furzeri provide a unique opportunity to study the relationship between diet, brain plasticity, and metabolism, making this fish a suitable model for aging research. In this study, we investigated the effects of different dietary regimens on the neuronal and metabolic response in Nothobranchius furzeri, focusing on the expression of markers related to neuronal activity (pS6), satiety regulation (Nesfatin), and glial response (GFAP).