Anticancer Effect of Minor Phytocannabinoids in Preclinical Models of Multiple Myeloma

Introduction: Multiple Myeloma (MM) is a blood cancer associated with severe bone-related complications included the myeloma cells ability of infiltrating the bone marrow and inducing osteoclast activation. Objective: Cannabigerol (CBG), Cannabichromene (CBC), Cannabinol (CBN) and Cannabidivarin (CBDV) were investigated in human MM in vitro and in vivo models. Materials & Methods: Cell viability, invasion and cell death assay, western blot analysis and bone resorption assay were performed on human MM cell lines (U266, RPMI, SKO), osteoblast cell line (HuOB), and THP-1 derived osteoclast. Moreover, in vivo analyses were performed also on MM xenograft mice model. Results: Phytocannabinoids inhibited the growth of MM cells by inducing necrotic cell death; they also reduced the MM cells bone invasion and bone resorption in vitro. CBN reduced the tumour mass in vivo. Conclusion: Our results showed that the phytocannabinoids studied potentially have antimyeloma activities.