Abstract Dietzia B32T was isolated from subcutaneous nodules of a 12-year-old mare in Reggio Calabria, Italy. Based on previously MALDI-TOF MS identification and studies using 16S rRNA gene sequence similarity, the B32T strain was confirmed as belonging to the genus Dietzia and most closely related to Dietzia lutea (97.07%). Objectives To describe the Dietzia B32T strain within the genus, morphological, biochemical, physiological, genomic analyses, and antibiotic susceptibility testing, were performed. Methods For the study, the following tests were conducted: Gram staining, and motility by optical and transmission microscopes; growth temperature (4-50 °C), growth curve at 37 °C using Thermo-Spectrophotometer Multiskan SkyHi-UV-Vis Reader; NaCl tolerance and haemolytic activity using liquid medium and BHI plates. For biochemical tests (catalase, glucose, sucrose, urea, nitrate reduction tests) the kit RapID™ CB-Plus (Remel ThermoFisher) was used. Susceptibility to Gentamicin-CN10, Ampicillin-AMP10, Tetracycline-TE30, Chloramphenicol-C30, Vancomycin-VA30, Clindamycin-CD2, Streptomycin-S10, Erythromycin-E15, was evaluated using Kirby Bauer method. In addition, sequencing (Illumina Hiseq) and complete genome assembly (FastQC-v.0.11.5, Edena-3.0) used as an external assembly for tree inference was performed. Digital values of DNA-DNA hybridization and mean nucleotide identity were calculated using the Genome-to-Genome Distance Calculator-2.1 (Leibniz-Institute DSMZ) and JSpecies Web Server, respectively. Results On the basis of biochemical, morphological, and genotypic data, the Dietzia B32T strain formed a monophyletic clade with the known species of this genus and represents a new species for which the name Dietzia calabriae sp. nov. has been proposed. Conclusions The genome of Dietzia calabriae sp. nov. is available in the GenBank database under accession number CP093845.
DIETZIA CALABRIAE SP. NOV.: FIRST CHARACTERIZATION OF AN ANIMAL BACTERIAL SPECIES
A. -R. Attili
Primo
;V. Cuteri;
2023-01-01
Abstract
Abstract Dietzia B32T was isolated from subcutaneous nodules of a 12-year-old mare in Reggio Calabria, Italy. Based on previously MALDI-TOF MS identification and studies using 16S rRNA gene sequence similarity, the B32T strain was confirmed as belonging to the genus Dietzia and most closely related to Dietzia lutea (97.07%). Objectives To describe the Dietzia B32T strain within the genus, morphological, biochemical, physiological, genomic analyses, and antibiotic susceptibility testing, were performed. Methods For the study, the following tests were conducted: Gram staining, and motility by optical and transmission microscopes; growth temperature (4-50 °C), growth curve at 37 °C using Thermo-Spectrophotometer Multiskan SkyHi-UV-Vis Reader; NaCl tolerance and haemolytic activity using liquid medium and BHI plates. For biochemical tests (catalase, glucose, sucrose, urea, nitrate reduction tests) the kit RapID™ CB-Plus (Remel ThermoFisher) was used. Susceptibility to Gentamicin-CN10, Ampicillin-AMP10, Tetracycline-TE30, Chloramphenicol-C30, Vancomycin-VA30, Clindamycin-CD2, Streptomycin-S10, Erythromycin-E15, was evaluated using Kirby Bauer method. In addition, sequencing (Illumina Hiseq) and complete genome assembly (FastQC-v.0.11.5, Edena-3.0) used as an external assembly for tree inference was performed. Digital values of DNA-DNA hybridization and mean nucleotide identity were calculated using the Genome-to-Genome Distance Calculator-2.1 (Leibniz-Institute DSMZ) and JSpecies Web Server, respectively. Results On the basis of biochemical, morphological, and genotypic data, the Dietzia B32T strain formed a monophyletic clade with the known species of this genus and represents a new species for which the name Dietzia calabriae sp. nov. has been proposed. Conclusions The genome of Dietzia calabriae sp. nov. is available in the GenBank database under accession number CP093845.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
