Bacterial translation initiation pathway entails several steps kinetically controlled by initiation factors IF1, IF2 and IF3. The pathway begins with formation of the 30S pre-initiation complex containing all 30S ligands followed by a locking step stabilizing the P-site decoding of the mRNA initiation triplet (AUG in most cases) by fMet-tRNAfmet recruited by 30S-bound IF2 through interaction of its C-domain with the initiator tRNA 3′acceptor-end. The 30S initiation complex thus formed is joined with the 50S subunit yielding a 70S-initiation complex competent in catalyzing peptide-bond formation and starting the elongation phase of translation. Different mRNA types exist with or without a 5′UTR and a Shine-Dalgarno sequence. Keywords: Bacteria; FMet-tRNA; GTPase; IF1; IF2; IF3; MRNA; PpGpp; Protein synthesis; Ribosomal subunits; RRNA ribosomes; Translation fidelity
Translation | Translation initiation in bacteria: Factors and mechanisms
Giuliodori A. M.
2021-01-01
Abstract
Bacterial translation initiation pathway entails several steps kinetically controlled by initiation factors IF1, IF2 and IF3. The pathway begins with formation of the 30S pre-initiation complex containing all 30S ligands followed by a locking step stabilizing the P-site decoding of the mRNA initiation triplet (AUG in most cases) by fMet-tRNAfmet recruited by 30S-bound IF2 through interaction of its C-domain with the initiator tRNA 3′acceptor-end. The 30S initiation complex thus formed is joined with the 50S subunit yielding a 70S-initiation complex competent in catalyzing peptide-bond formation and starting the elongation phase of translation. Different mRNA types exist with or without a 5′UTR and a Shine-Dalgarno sequence. Keywords: Bacteria; FMet-tRNA; GTPase; IF1; IF2; IF3; MRNA; PpGpp; Protein synthesis; Ribosomal subunits; RRNA ribosomes; Translation fidelityI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
