Parathyroid tumours are heterogeneous and parathyroid carcinoma (PC) is a rare endocrine malignancy, accounting for less than 1% of all cases of sporadic primary hyperparathyroidism. In clinical practice, PC diagnosis is challenging and in the majority of cases is made postoperatively at histology. Here, we report the case of a Caucasian man who presented an adenoma and a carcinoma localized in two different parathyroid glands. After surgical removal, tumour tissues were processed to obtain proteome profiles. Briefly, 40 µg aliquots of protein extracts from both adenoma and carcinoma samples were treated with cyanine dyes (Cy5, Cy2, and Cy3) and separated using Differential Gel Electrophoresis (DIGE). Comparative analysis resulted in 37 differentially expressed spots. For mass spectrometry identification, a preparative 2-DE was performed, gel was silver stained, protein spots were digested by trypsin and resulting peptides analyzed by LC-MS/MS. Among 37 differentially expressed proteins, 31 showed an increased expression in carcinoma compared to adenoma. The most significant proteins were Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), Annexin A4 (ANXA4) and Chloride intracellular channel protein 1 (CLIC1) with fold change of 6, 3 and 2.3, respectively. Previous immunohystochemical studies have suggested UCHL1 together with parafibromin as markers of parathyroid carcinoma. Our proteomic results, obtained in the same patient for two different tumours, are a strong evidence in favour of UCHL1 and other proteins as candidate positive markers in differential diagnosis of parathyroid tumours. The use of proteomic can increase the knowledge of the molecular steps involved in PC pathogenesis.
Comparative Protein Profile of Parathyroid Adenoma and Carcinoma: a Case Report
Laura GiustiUltimo
2019-01-01
Abstract
Parathyroid tumours are heterogeneous and parathyroid carcinoma (PC) is a rare endocrine malignancy, accounting for less than 1% of all cases of sporadic primary hyperparathyroidism. In clinical practice, PC diagnosis is challenging and in the majority of cases is made postoperatively at histology. Here, we report the case of a Caucasian man who presented an adenoma and a carcinoma localized in two different parathyroid glands. After surgical removal, tumour tissues were processed to obtain proteome profiles. Briefly, 40 µg aliquots of protein extracts from both adenoma and carcinoma samples were treated with cyanine dyes (Cy5, Cy2, and Cy3) and separated using Differential Gel Electrophoresis (DIGE). Comparative analysis resulted in 37 differentially expressed spots. For mass spectrometry identification, a preparative 2-DE was performed, gel was silver stained, protein spots were digested by trypsin and resulting peptides analyzed by LC-MS/MS. Among 37 differentially expressed proteins, 31 showed an increased expression in carcinoma compared to adenoma. The most significant proteins were Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), Annexin A4 (ANXA4) and Chloride intracellular channel protein 1 (CLIC1) with fold change of 6, 3 and 2.3, respectively. Previous immunohystochemical studies have suggested UCHL1 together with parafibromin as markers of parathyroid carcinoma. Our proteomic results, obtained in the same patient for two different tumours, are a strong evidence in favour of UCHL1 and other proteins as candidate positive markers in differential diagnosis of parathyroid tumours. The use of proteomic can increase the knowledge of the molecular steps involved in PC pathogenesis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
