Introduction: Positive effect of L-Carnitine supplementation in cardiovascular diseases has been recently questioned due to the evidence on trimethylamine-N-oxide (TMAO) production by gut microbiota. Objectives: The aim of this study was to evaluate the impact of L-carnitine supplementation on TMAO level, lipid profile and platelets mitochondrial DNA methylation (mtDNA) at two selected regions (MTCO1, D-loop) in a group of aged women that conducted a resistance training protocol (24-weeks, twice a week, with or without supplementation). Material and Methods: The study protocol has been approved by the Independent Bioethics Commission for Research at Medical University of Gdansk (NKBBN/354- 201/2017). Thirty women, from 62 to 72 years old were analysed in the study. Plasma TMAO (ng/ml) was determined by the UPLC/MS/MS method. Platelet mtDNA extraction was performed using the Genomic DNA extraction kit (Norgen Biotek Corp., Canada). MtDNA of MTCO1 and D-loop was measured, after the conversion of mtDNA with bisulfite, by the PyroMark PCR Kit (Qiagen, Germany). Lipid profile was measured by automatic analyzer Cobas6000. Results: Data analyses revealed that L-Carnitine supplementation significantly modify platelet mtDNA methylation at D-loop region and this change was associated with an improved lipid profile. Conclusions: A complex correlation among TMAO, mtDNA and lipid profile suggests that L-carnitine supplementation together with resistance training activity can exert a positive effect on cardiovascular health. This study was supported by the National Science Centre in Poland, grant number 2014/15/B/NZ7/00893 and RG’s Institutional research fund-Unicam (FPA000033).
Platelet mitochondrial DNA methylation in L-carnitine supplemented aged women.
Bordoni L.;Gabbianelli R.
2019-01-01
Abstract
Introduction: Positive effect of L-Carnitine supplementation in cardiovascular diseases has been recently questioned due to the evidence on trimethylamine-N-oxide (TMAO) production by gut microbiota. Objectives: The aim of this study was to evaluate the impact of L-carnitine supplementation on TMAO level, lipid profile and platelets mitochondrial DNA methylation (mtDNA) at two selected regions (MTCO1, D-loop) in a group of aged women that conducted a resistance training protocol (24-weeks, twice a week, with or without supplementation). Material and Methods: The study protocol has been approved by the Independent Bioethics Commission for Research at Medical University of Gdansk (NKBBN/354- 201/2017). Thirty women, from 62 to 72 years old were analysed in the study. Plasma TMAO (ng/ml) was determined by the UPLC/MS/MS method. Platelet mtDNA extraction was performed using the Genomic DNA extraction kit (Norgen Biotek Corp., Canada). MtDNA of MTCO1 and D-loop was measured, after the conversion of mtDNA with bisulfite, by the PyroMark PCR Kit (Qiagen, Germany). Lipid profile was measured by automatic analyzer Cobas6000. Results: Data analyses revealed that L-Carnitine supplementation significantly modify platelet mtDNA methylation at D-loop region and this change was associated with an improved lipid profile. Conclusions: A complex correlation among TMAO, mtDNA and lipid profile suggests that L-carnitine supplementation together with resistance training activity can exert a positive effect on cardiovascular health. This study was supported by the National Science Centre in Poland, grant number 2014/15/B/NZ7/00893 and RG’s Institutional research fund-Unicam (FPA000033).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
