Japanese Encephalitis Virus (JEV) can be responsible for encephalitis in humans and horses and for reproductive disorders in pigs. Birds are amplifying hosts and JEV has been detected in different organs. There are no published data showing the presence of the virus in the organs of healthy birds, captured during inter epidemic periods. We report the results obtained from samples from 12 young birds collected in Italy in 2011 - 2012. Serial sections from formalin-fixed and paraffinembedded tissue samples were routinely processed for histopathology, immunohistochemistry (IHC; pAb, PG 10004, Genesis Biotech Inc.) and Sybr Green real time PCR. IHC to JEV was positive in 9 of 12 bone marrows, showing reaction products in stem cells concurrently positive also for anti CD11b antibody. Four of 12 bone marrows were positive by real time PCR, while other samples showed low quality nucleic acids. JEV localization in bird’s bone marrow myeloid lineage cells, suggest that monocytemacrophage precursors may represent a target cell for the virus, as recently demonstrated in the arbovirus Chikungunya virus. Our results suggest that birds could be reservoir for JEV maintaining myeloid cells persistently infected with the virus.
3rd International Conference on Avian heRpetological and Exotic mammal medicine
PREZIUSO, Silvia;MARI, Subeide;ROSSI, Giacomo
2017-01-01
Abstract
Japanese Encephalitis Virus (JEV) can be responsible for encephalitis in humans and horses and for reproductive disorders in pigs. Birds are amplifying hosts and JEV has been detected in different organs. There are no published data showing the presence of the virus in the organs of healthy birds, captured during inter epidemic periods. We report the results obtained from samples from 12 young birds collected in Italy in 2011 - 2012. Serial sections from formalin-fixed and paraffinembedded tissue samples were routinely processed for histopathology, immunohistochemistry (IHC; pAb, PG 10004, Genesis Biotech Inc.) and Sybr Green real time PCR. IHC to JEV was positive in 9 of 12 bone marrows, showing reaction products in stem cells concurrently positive also for anti CD11b antibody. Four of 12 bone marrows were positive by real time PCR, while other samples showed low quality nucleic acids. JEV localization in bird’s bone marrow myeloid lineage cells, suggest that monocytemacrophage precursors may represent a target cell for the virus, as recently demonstrated in the arbovirus Chikungunya virus. Our results suggest that birds could be reservoir for JEV maintaining myeloid cells persistently infected with the virus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
