EFFICACY OF TOPICAL APPLICATION OF KLOX BIOPHOTONIC SYSTEM IN CANINE AND FELINE ACUTE SIMPLE SURGICAL AND CHRONIC WOUNDS. PRELIMINARY RESULTS

Cutaneous lesions, whether of an ulcerative or dehiscent character, often need a complex clinical management, not just for the difficult and slow healing, but also for managing the possible secondary complications that can result in infection, physical pain, emotional distress to the patient and lengthy hospitalization. In recent years, KLOX Technologies Inc. has developed an innovative technology platform, the KLOX B op oton Syst m ―KLOX‖ w ons sts of pr m ry mult -LED light device and a topical photo-converter gel, with applications in dermatology and wound treatment. The aim of this research is to investigate whether the KLOX treatment promotes and accelerates the healing process and reduces bacterial contamination in canine and feline ut sur l n ron woun s Pr l m n ry r sults of t f rst y r‘s r s r re presented.Two groups of patients are part of this study: - Dogs and cats having orthopedic surgeries (Group 1). The KLOX treatment was applied every 3 days for 12 days, on the proximal half of the surgical wound. As a control, the distal half of the wound received just saline solution. For patients with bilateral surgical wounds, one was treated with KLOX and the other only with saline. Some patients were subjected to cutaneous and subcutaneous biopsy of the proximal district (KLOX) and the distal district (saline). - Dogs and cats with delayed healing of surgical wounds, protracted decubital ulcers, burns or traumatic wounds (Group 2). KLOX was applied every 3 days either on 1⁄2 or on all of the lesion (if the patient has more lesions), until complete re-epithelialization. Each application was documented photographically with a millimeter reference scale, and by using Photoshop extended, enabling the degree of tissue regeneration to be measured through the calculation of the wound area. During the different treatment steps they were performed swabs for bacterial cultures. To date, 25 patients have been treated (12 Group 1 & 13 Group 2); the study has shown a very good regeneration of chronic wounds (Group 2), with a first phase of intense granulation coming from the bottom of the wound (vertical regeneration), followed by a skin growth with wedges to about 6 days from the first treatment (horizontal regeneration). The intense production of granulation tissue has been modulated, in a second time, with a reduction of its volume; therefore, no patient developed exuberant scars or keloids. The regeneration of all the tissue layers involved in the disease process occurred faster versus wounds treated with traditional methods (control). The histological results of biopsies performed on KLOX versus control-treated portions of surgical wounds (Group 1) indicated that the KLOX-treated tissue had better and complete re-epithelialization, minor inflammation of the dermal layer, less neoangiogenesis and the presence of synthesis activities of the connective matrix. Preliminary results showed the efficacy of the KLOX treatment to promote tissue regeneration and inhibit bacterial growth. Further studies will be needed to give strength to this initial research.