Clam Scapharca Inaequivalvis is a mollusk of Indo-Pacific origin, currently living also in the Adriatic Sea, where it is considered undesirable since fishermen must pick it out from the clam Camelea Gallina.1 A possible valorization of this product is to use it as source of polyunsaturated fatty acids (PUFA) having a well known beneficial influence on the vascular system2. Thus, our study aimed at evaluating the efficiency of several extraction methods toward the total lipids and toward the individual fatty acids classes (saturated, monounsaturated and polyunsaturated). Conventional methods as Folch procedure3, Bligh & Dyer4, Soxhlet extraction were tested, but also accelerated solvent extraction (ASE) and the use of solvents with low toxicity were evaluated. The highest yields of total lipid extract were obtained using ASE with CHCl3/MeOH and Folch procedure, giving respectively 1.5 and 1.3 % expressed as average on the fresh sample, while the lowest yields were afforded by hexane/i-PrOH and Soxhlet with hexane giving 0.5 % of extract. The fatty acids composition of each lipid extract is being currently investigated. Results and methodology will be presented and discussed.
COMPARISON OF METHODS FOR THE EXTRACTION OF LIPIDS FROM CLAM SCAPHARCA INAEQUIVALVIS
FIORINI, Dennis;PETRINI, Agnese;GIGLI, Flavia;BALLINI, Roberto
2011-01-01
Abstract
Clam Scapharca Inaequivalvis is a mollusk of Indo-Pacific origin, currently living also in the Adriatic Sea, where it is considered undesirable since fishermen must pick it out from the clam Camelea Gallina.1 A possible valorization of this product is to use it as source of polyunsaturated fatty acids (PUFA) having a well known beneficial influence on the vascular system2. Thus, our study aimed at evaluating the efficiency of several extraction methods toward the total lipids and toward the individual fatty acids classes (saturated, monounsaturated and polyunsaturated). Conventional methods as Folch procedure3, Bligh & Dyer4, Soxhlet extraction were tested, but also accelerated solvent extraction (ASE) and the use of solvents with low toxicity were evaluated. The highest yields of total lipid extract were obtained using ASE with CHCl3/MeOH and Folch procedure, giving respectively 1.5 and 1.3 % expressed as average on the fresh sample, while the lowest yields were afforded by hexane/i-PrOH and Soxhlet with hexane giving 0.5 % of extract. The fatty acids composition of each lipid extract is being currently investigated. Results and methodology will be presented and discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
