transmission of scent information and are located in the subcutaneous layer of the carpus caudomedial surface. Their histological and histochemical features have been extensively investigated in both domestic and wild pigs and have provided important information regarding secretion chemical characteristics. The aim of the present work is to highlight the presence of leptin and its receptor in these glands and to verify the possibility of difference expressions between wild and domestic subjects, with the pourpose of providing data useful to better understand the mechanisms regulating their functionality. Eight clinically healthy adult male pigs, four domestic and four wild, were used. Carpal gland specimens were obtained immediately after slaughter, fixed in 4% formaldehyde solution and subsequently processed for embedding in paraffin, following routine tissue preparation procedures. The immunohistochemical reaction was visualized, utilising the avidin!biotin!complex and the DAB as the chromogen. The following primary antibodies were used: anti!Ob rabbit polyclonal antibody and anti!Ob!R goat polyclonal antibody. The immunohistochemical study showed a strong positivity for leptin and its receptor in the carpal glands of the animals examined. In particular, the immuno!reaction seems to affect only the cytoplasm of the dark cells while the clear ones are always negative. Immuno!positivity for leptin and its receptor was not observed in the epithelium of the ducts or in the connective tissue. In addition, there were no appreciable differences between domestic and wild swine. Recent studies conducted on some exocrine glands have allowed researchers to highlight how the same are able to secrete leptin and may represent a target of the action of this protein. This has enabled them to understand how the action of the Ob protein is much more extensive than first imagined. In this sense the results obtained in the present study confirm what has previously been stated and allow us to hypothesize that this gland is able to produce leptin and is itself a target of this molecule that may act by in autocrine/paracrine manner without being influenced by the different conditions of life.
LEPTIN AND ITS RECEPTOR IN WILD AND DOMESTIC SWINE CARPAL GLANDS
SCOCCO, Paola;
2013-01-01
Abstract
transmission of scent information and are located in the subcutaneous layer of the carpus caudomedial surface. Their histological and histochemical features have been extensively investigated in both domestic and wild pigs and have provided important information regarding secretion chemical characteristics. The aim of the present work is to highlight the presence of leptin and its receptor in these glands and to verify the possibility of difference expressions between wild and domestic subjects, with the pourpose of providing data useful to better understand the mechanisms regulating their functionality. Eight clinically healthy adult male pigs, four domestic and four wild, were used. Carpal gland specimens were obtained immediately after slaughter, fixed in 4% formaldehyde solution and subsequently processed for embedding in paraffin, following routine tissue preparation procedures. The immunohistochemical reaction was visualized, utilising the avidin!biotin!complex and the DAB as the chromogen. The following primary antibodies were used: anti!Ob rabbit polyclonal antibody and anti!Ob!R goat polyclonal antibody. The immunohistochemical study showed a strong positivity for leptin and its receptor in the carpal glands of the animals examined. In particular, the immuno!reaction seems to affect only the cytoplasm of the dark cells while the clear ones are always negative. Immuno!positivity for leptin and its receptor was not observed in the epithelium of the ducts or in the connective tissue. In addition, there were no appreciable differences between domestic and wild swine. Recent studies conducted on some exocrine glands have allowed researchers to highlight how the same are able to secrete leptin and may represent a target of the action of this protein. This has enabled them to understand how the action of the Ob protein is much more extensive than first imagined. In this sense the results obtained in the present study confirm what has previously been stated and allow us to hypothesize that this gland is able to produce leptin and is itself a target of this molecule that may act by in autocrine/paracrine manner without being influenced by the different conditions of life.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
