Donkey milk caseins display great microheterogeneity by two-dimensional electrophoresis (2-DE), probably because of a variable degree of phosphorylation and/or alternative splicing phenomena. In this work, we have investigated the complexity of the donkey milk caseins: The whole casein fraction was subjected to treatment with calf intestinal alkaline phosphatase to achieve dephosphorylation. The obtained apo forms were analyzed by 2-DE and compared with whole donkey caseins. As a result, donkey milk β-casein and its splicing variants are present as different phosphorylated isoforms, whereas α S1-casein displays several isoforms that are derived from both phosphorylation and glycosylation phenomena.

Effect of Dephosphorylation on Donkey Milk Caseins

VINCENZETTI, Silvia;VITA, Alberto;CARPI, FRANCESCO MARTINO;POLIDORI, Paolo
2013-01-01

Abstract

Donkey milk caseins display great microheterogeneity by two-dimensional electrophoresis (2-DE), probably because of a variable degree of phosphorylation and/or alternative splicing phenomena. In this work, we have investigated the complexity of the donkey milk caseins: The whole casein fraction was subjected to treatment with calf intestinal alkaline phosphatase to achieve dephosphorylation. The obtained apo forms were analyzed by 2-DE and compared with whole donkey caseins. As a result, donkey milk β-casein and its splicing variants are present as different phosphorylated isoforms, whereas α S1-casein displays several isoforms that are derived from both phosphorylation and glycosylation phenomena.
2013
9783642364877
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/270583
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? ND
social impact