The applicability of some assays to evaluate the effectiveness of the immune function, as previously used in other animal species (pigs, cows and sheep), was tested in an alpaca (Lama pacos) herd affected by Caseous Lymphadenitis (Corynebacterium pseudotubercolosis) and subject to a vaccination programme against the causative agent. The vaccination programme was initiated following failure of controlling the outbreak by isolation and treatment of the initially affected animals. An homotypic, autogenous inactivated and adjuvated (aluminium hydroxide) vaccine, administered subcutaneously at day 0 (A) and after 21 days (B) was used. Blood samples were collected by venipuncture of the jugular vein on days 0, 21 and 42 (C). Samples in EDTA, collected on day A and C, were analysed for standard haematological parameters. Serum obtained from whole blood with no anticoagulant on days A, B and C was tested for: Lysozyme serum titration (expressed in mu g/m), serum Bactericidal assay (expressed in %), semiquantitative complement titration (expressed in CH50) and immunoglobuline (by electrophoresis) and IgGs concentrations (by Radial Immunodiffusion tests).

EVALUATION OF THE IMMUNE RESPONSE TO VACCINATION AGAINST C. PSEUDOTUBERCOLOSIS IN AN ALPACA HERD IN ITALY: PRELIMINARY RESULTS.

BEGHELLI, Daniela;
2006

Abstract

The applicability of some assays to evaluate the effectiveness of the immune function, as previously used in other animal species (pigs, cows and sheep), was tested in an alpaca (Lama pacos) herd affected by Caseous Lymphadenitis (Corynebacterium pseudotubercolosis) and subject to a vaccination programme against the causative agent. The vaccination programme was initiated following failure of controlling the outbreak by isolation and treatment of the initially affected animals. An homotypic, autogenous inactivated and adjuvated (aluminium hydroxide) vaccine, administered subcutaneously at day 0 (A) and after 21 days (B) was used. Blood samples were collected by venipuncture of the jugular vein on days 0, 21 and 42 (C). Samples in EDTA, collected on day A and C, were analysed for standard haematological parameters. Serum obtained from whole blood with no anticoagulant on days A, B and C was tested for: Lysozyme serum titration (expressed in mu g/m), serum Bactericidal assay (expressed in %), semiquantitative complement titration (expressed in CH50) and immunoglobuline (by electrophoresis) and IgGs concentrations (by Radial Immunodiffusion tests).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11581/115607
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