In Italy, according to the European directives, the diagnosis of tuberculosis in cattle is based on intradermal inoculation of bovine tuberculin (5,000 IU of PPD in 0,1 ml) and/or inspection during slaughtering. The reintroduction of infections observed in some free-areas suggested to investigate wild animals in order to verify their role in bovine tuberculosis epidemiology. A two years study has been carried out in wild boars reared in free range conditions in four different regions of Central Italy. Retro-phariyngeal and sub-mandibular lymph nodes from 336 animals without macroscopic lesion were examined for mycobacteria and 16 samples (4,8 %) resulted positive at cultures . All the isolated mycobacteria were submitted to biochemical test and randomly for PCR. On the basis of the metabolic tests, the isolates were not identified as Mycobacterium bovis, and according to the PCR results,all of theme were belonging to genus Mycobacterium. According to these results, the wild boars seem to be susceptible to mycobacteria different from M. bovis. Since infected wild boars could live in the same habitat with cows, they could be considered rather as markers of mycobacteria infection in the environment than M. bovis reservoirs. These findings suggest the need to keep in mind the presence of such boars where tuberculosis he's been previously eradicated and were doubtful cases of skin test are detected.
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|Titolo:||Epidemiological role of wild boars in bovine tuberculosis reintroduction.|
|Data di pubblicazione:||2005|
|Appare nelle tipologie:||Contributo in atto di convegno su volume|